摘要
目的分析CKl9、Galectin-3、HBMEl、CyclinDt及TPO蛋白在甲状腺乳头状癌及结节性甲状腺肿伴良性乳头状增生中的表达及其在诊断与鉴别诊断中的价值。方法采用免疫组化EnVision法检测60例甲状腺乳头状癌与56例结节性甲状腺肿伴良性乳头状增生中CK19、Galectin3、HBME1、CyclinD1及TPO的表达。结果CK19、Galectin3、HBMEl和CyclinDl在甲状腺乳头状癌中的阳性率分别是98．3％、98．3％、90．0％、76．7％，TPO的阴性率96．7％。而在结节性甲状腺肿伴良性乳头状增生中分别为21．4％、17．8％、1．8％和66．1％，TPO的阴性率为1．8％。除CyclinDl外其他4种抗体在甲状腺乳头状癌与结节性甲状腺肿伴乳头状良性增生中两组之间的表达差异均有统计学意义（均P〈0．01）。CyclinD1在甲状腺良恶性病变中的表达阳性率分别为66．1％和76.17％，两者间差异无统计学意义（P〉0．05）。联合应用4种抗体在鉴别甲状腺乳头状癌与良性病变时的敏感度、特异度、准确度分别为95％、98．2％、96．6％。结论CK19、Galectin3、HBMEl与CyclinDl用于甲状腺乳头状癌诊断的阳性率高于结节性甲状腺肿伴良性乳头状增生，可作为甲状腺乳头状癌诊断的重要标记物，而TPO的升高可作为结节性甲状腺肿伴良性乳头状增生诊断的重要标记物。同时，4抗体在良恶性乳头状增生中阳性率的差异有统计学意义，联合应用对甲状腺乳头状癌的诊断与鉴别诊断具有重要价值。
Objective To explore the expression of CK19, Galectin-3, HBME1, CyclinD1 and TPO protein and their diagnostic value in papillary thyroid carcinoma and benign papillary hyperplasia with nodular goiter. Methods En Vision immunostaining method was used to detect the expression of CK19, Galectin-3, HBME1, CyclinD1 and TPO protein in 60 cases of papillary thyroid carcinoma and 56 cases of benign papillary hyperplasia with nodular goiter. Results In 60 cases of papillary thyroid carcinoma, the positive rate of CK19, Galectin-3, HBME1 and CyclinD1 was 98.3%, 98.3%, 90% and 76.7% respectively and the negative rate of TPO was 96.7%. By contrast, the positive rate of CK19, Galectin-3, HBME1 and CyclinD1 in benign papillary hyperplasia with nodular goiter was 21.4%, 17.8%, 1.8% and 66.1%, and the negative rate of TPO protein was 1.8%., The expression of the other four antibodies had significant difference （P〈0.01） between papillary thyroid carcinoma and benign papillary hyperplasia, except of CyclinD1. On the other hand, there was no significant difference of the expression of CyclinD1 bertween these two diseases （76.7% vs 66.1%, P 〉0.05）. The sensitivity, specificity and accuracy of the joint application of the four antibodies （CK19, Galectin-3, HBME1, and TPO protein） was 96.6%, 98.8%, 98%, respectively in differentiated diagnosis between papillary thyroid carcinoma and benign papillary hyperplasia with nodular goiter. Conclusion The expression of CK19, Galectin-3, HBME1 and CyclinD1 is significantly higher in papillary thyroid carcinoma than benign papillary hyperplasia with nodular goiter. The former three antibodies might be used as important markers for pathological diagnosis of papillary thyroid carcinoma, and the joint application of these four antibodies might be of important value in the pathological diagnosis and differentiated diagnosis of papillary thyroid carcinoma. While the expression of TPO might be important marker for benign papillary hyperplasia with nodular goiter.
出处
《浙江医学》
CAS
2012年第20期1650-1653,共4页
Zhejiang Medical Journal
