摘要
目的 应用克隆形成方法 ,研究羟基喜树碱 (HCPT)对人鼻咽癌细胞系 (CNE)和胃癌细胞系 (BGC 82 3)的放射增敏作用。方法 实验分为单纯照射组和照射加药组。照射加药组在照射后均立即给予HCPT 2 μg/ml(药物剂量为ID50 剂量 ) ,37℃孵箱内作用 4h。应用克隆形成方法 ,观察单纯照射和照射加HCPT对细胞的杀伤作用。计算细胞存活率 ,用单击多靶数学模型进行曲线拟合做图。结果 BGC 82 3细胞单纯照射组D0 值为 1 17Gy,Dq 值为 1 91Gy,N值为 5 14;照射加HCPT组D0 值为 0 95Gy ,Dq 值为 0 0 1Gy ,N值为 1 0 1;放射增敏比 (SER)为 1 2 3(1 17/ 0 95 )。CNE Ⅰ细胞单纯照射组D0 值为 1 6 0Gy ,Dq 值为 0 6 5Gy ,N值为 1 5 ;照射加HCPT组D0 值为 0 95Gy ,Dq 值为 0 0 1Gy ,N值为 1 0 1;SER为 1 6 8(1 6 / 0 95 )。结论 研究结果显示 ,HCPT具有一定的放射增敏作用 ,为临床的放疗和HCPT的联合应用提供了实验依据。
Objective To study the radiation sensitizing effect of hydroxycamptothecin (HCPT) on human stomach adenocacinoma and nasopharyngeal carcinoma.Methods Human stomach cancer cell line BGC823 and nasopharyngeal cancer cell line CNE 1 were treated in vitro with different doses of radiation alone or radiation followed by HCPT (2 μg/ml) for 4 h. Colony forming assay was used for survival fraction analysis. Single hit multitarget model was used to plot survival curve.Results The value of D 0, D q and N of BGC823 cells received radiation treatment alone was 1.17 Gy, 1.91 Gy and 5.14 respectively. That of BGC 823 cells received radiation plus HCPT was 0.95 Gy, 0.01 Gy and 1.01 respectively. The sensitization enhanecment (SER) ratio was 1.23(1.17/0.95). Similar results were obtained for CNE 1 cells treated with radiation alone or in combination with HCPT, with an SER of 1.68.Conclusion HCPT has been shown a radiosensitizing effect in vitro on gastric and nasopharyngeal cancer cells. Subject
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2000年第2期124-126,共3页
Chinese Journal of Oncology
