摘要
目的 探讨人微小病毒B19(ParvovirusB19)、弓形体 (Toxoplasma,TOX)、风疹病毒 (Rubellavirus,RV)、巨细胞病毒 (Cytomegalovirus ,CMV)和单纯疱疹病毒 2型 (Herpessimplexvirustype 2 ,HSV2 )的感染与先天性心脏病的关系。方法 采用病例对照研究 ,以先天性心脏病心肌组织及正常心脏心肌组织为标本 ,用PCR或RT PCR技术进行病毒基因的调查。结果 在 6 6例先天性心脏病心肌组织中检测到B19、TOX、CMV和HSV2的阳性率分别为 18 2 %、15 2 %、2 5 8%和 4 5 %。 38例对照中B19、TOX、CMV和HSV2的阳性率分别为 0 %、3%、2 2 %和 3%。B19、TOX在两组中检测差异有显著性 (P <0 0 5 ) ,CMV、HSV2在两组中无统计学意义 (P >0 0 5 )。在 30例先天性心脏病中RV阳性率 2 3 3% ,2 0例对照中均阴性 ,两组间差异有显著性 (P =0 0 32 8)。结论 在先天性心脏病心肌组织中检测到了B19、TOX、RV、CMV和HSV2基因 ,调查表明 ,B19、RV和TOX的感染是重要的危险因子 ,它可能与先天性心脏病的发病有关 ,CMV。
Objective To explore relationship of Parvovirus B19(B19), Toxoplasma gondii(TOX), rubella virus(RV), cytomegalovirus (CMV) or herpes simplex virus type 2 (HSV 2) infection with congenital heart disease (CHD). Methods We conducted a case control study on detection of B19, TOX, RV, CMV or HSV 2 gene in cardiac tissue of 66 cases of CHD and 38 cases controls with polymerase chain reaction (PCR). Results 1) The positive rates of B19, TOX, CMV and HSV 2 in 66 cases CHD were 18.2%, 15.2%, 25.8% and 4.5% , respectively, while that in 38 cases control groups were 0%, 2.6%, 21.2% and 2.6%, reapectively. The positively of B19, TOX was significantly different ( P <0.05) between CHD and control groups, while no statistical difference ( P <0.05) for CMV and HSV 2. 2) Seven of 30 (23.3%) CHD were positive for RV RNA, compared with control group which was all negative ( P =0.0327). Conclusion The results show that B19, TOX, RV infection mighet be important risk factors for CHD, while CMV and HSV 2 had no relationship with CHD.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2000年第2期170-172,共3页
Chinese Journal of Microbiology and Immunology
基金
国家计生委基金资助项目! (9736)
关键词
先天性心脏病
微小病毒
弓形体
风疹病毒
Congenital heart disease
Parrovirus B19
Toxoplasma
Rubellavirus
Cytomegalovirus
Herpes simplex virus
