摘要
目的分析精子DNA完整性与精液主要参数的相关性，探讨其在男性不育诊断中的临床应用价值。方法收集2010年6月至2012年2月在门诊就诊的465例男性患者的精液标本，参照第5版《世界卫生组织人类精液检查与处理实验室手册》严格标准进行精液常规及精子形态学分析，然后采用精子染色质扩散法检测精子DNA完整性，计算DNA碎片指数（DNA fragmentation index，DFI），按DFI值分为3组，分别为A组DFI≤15％（n=183）、B组15％〈DFI〈30％（n=208）和C组DFI≥30％（n=74）。系统分析精子DFI与精液主要参数的相关性。结果（1）各组间精液圆细胞浓度、精子活力、活动率及正常形态率差异有统计学意义（P〈0．05）；（2）各组间的精子总数差异无统计学意义（P〉0．05）。结论精子DNA完整性检测结合精液常规分析有助于评估男性精液质量及生育力。
Objective To investigate the correlations between sperm DNA integrity and semen parameters, and evaluate sperm DNA integrity testing in diagnosis of male infertility. Methods Semen samples from 465 male patients were collected at our department from June 2010 to February 2012. The standard routine semen and morphological analysis were done based on the criteria of semen analysis handbook, 5thh edition of the World Health Organization. The technique of sperm chromatin dispersion （SCD） was used to detect the percentage of spermatozoa DNA damage, and the sperm DNA fragmentation index （DFI） was also calculated. All the samples were divided into three groups-Group A： DFI≤ 15% （n=183）, Group B： 15%〈DFI〈30% （n=208） and Group C： DFI ≥30%（n=74）. The correlation between the sperm DNA fragmentation index（DFI） and the sperm parameters were analyzed. Results （1） There were significant differences among the three groups in semen oval cell consistence, sperm motility, viability, and rate of normal morphology （P〈0.05） .（2） There was no difference in sperm number among the three groups（P〉0.05）. Conclusion The sperm DFI testing combined with the routine semen analysis is better than exclusive semen parameters in assessment of the spern quality and male fertility.
出处
《中国男科学杂志》
CAS
CSCD
北大核心
2012年第9期15-18,23,共5页
Chinese Journal of Andrology
基金
广西卫生厅科研课题（Z2012211,Z2012210）
