摘要
采用全细胞膜片钳技术研究β-受体激动剂异丙肾上腺素调节窦房结起搏细胞延迟整流钾通道信号传递机制。结果发现 ,采用 c AMP-依赖性蛋白激酶抑制剂 (H- 89)选择性阻断 c AMP-依赖性蛋白激酶磷酸化途径后 ,异丙肾上腺素依赖性 Ik 激活被抑制 ,而用选择性蛋白激酶 C抑制剂 Bisindolymaleim ide(磷酸化途径 )后则异丙肾上腺素依赖性 Ik 激活未能被抑制。提示异丙肾上腺素调节 Ik 的信号传递机制主要通过 c AMP-依赖性蛋白激酶 (PKA)磷酸化途径。
Signal transmit mechanism of β acceptor agonist isoprenaline regulating sinus P cell delayed rectifier potassium channels was studied by using whole cell patch clamp technique. It was found that the activation of isoprenaline dependent Ik was inhibited by using antagonist (H 89) of cAMP dependent PKA to block phosphorylation of cAMP dependence PKA. But the activation of isoprenaline dependent Ik was not inhibited by using selective antagonist (Bisindolymaleimide I) of PKA C. It was suggested that the signal transmit mechanism of isoprenaline regulating Ik might be mainly contributed to the phosphorylation of cAMP dependent PKA.
出处
《同济医科大学学报》
CSCD
2000年第2期122-123,126,共3页
Acta Universitatis Medicinae Tongji
