摘要
目的 探讨HPV16型DNA与结直肠癌临床病理和预后的关系。方法 结直肠癌 5 0例、腺瘤 38例和正常结直肠粘膜组织 2 0例 ,应用多聚酶链反应 (PCR)检测HPV16型DNA序列 ,并对其扩增产物进行电泳分析和southernblot分析。追踪观察患者的预后。结果 三组患者HPV16型DNA阳性率分别为 42 %、31 6 %和 0。结直肠癌和腺瘤分别与正常大肠粘膜组织比较 ,HPV16型DNA阳性率有显著性差异 (P <0 0 5 ) ;结直肠癌和腺瘤之间比较 ,HPV16型DNA阳性率无显著性差异 (P >0 0 5 )。HPV16型DNA阳性与结直肠癌患者的年龄、性别 ,以及肿瘤的部位、大小、组织类型、临床分期均无关 ,但与其预后有关。
Objective To assess if Human papillomavirus type16 DNA are associated with human colorectal adenocarcinoma in clinico pathologic characteristics and prognosis.Methods This paper used polymerase chain reaction(PCR) for the detection of HPV type 16 DNA in paraffin embeded tissue section of the patients with colorectal adenocarcinoma(50 cases),colorectal adenoma(38 cases)and normal colorectal tissue(20 cases).Amplified products were analyzed by agarose gel electrophoresis and Southern blot.Results The results showed that HPV type 16 DNA sequences were found in 42%(21/50) of colorectal adenocarcinoma and 31 6%(12/38) of colorectal adenoma.But,normal colorectal mucosal samples gained negative results.Comparison of colorectal adenocarcinoma(or colorectal adenoma)with normal colorectal mucosal samples,positive rates of HPV type 16 DNA were significantly higher( P <0 05).Positive rates of HPV type 16 DNA in coloretal adenoma were not clearly higher than in colorectal adenocarcinoma.There was no correlation between HPV type 16 DNA and the age,sex,tumor location,histological differentiation,or tumour stage of colorectal tumour.but correlation between it and prognosis.Conclusion The results implicate HPV type 16 may be one of index of prognosis in patients with colorectal adenocarcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2000年第2期109-111,共3页
Cancer Research on Prevention and Treatment
关键词
乳头瘤病毒
结肠癌
直肠癌
病理
聚合酶链反应
Human papapilloma virus(HPVs)
Colorectal carcinoma
Polymerase chain reaction(PCR)
Southern blot
