摘要
目的:分析浙南忍冬属药用植物叶绿体rbcL基因序列,探讨其差异。方法:Primer Premier5.0设计引物,用于rbcL基因PCR扩增,ClustalX1.83进行序列全比对。MEGA 4.0分析转换值、颠换值等;以七子花Heptacodium miconioides为外类群,邻接法(Neighbor-Joining,NJ)和最大简约法(Maximun Parsimony,MP)进行聚类分析(models:p-distance);自展法(Bootstrap)检验各分支可信度。结果:5种忍冬属药材rbcL基因片段长度在1 143～1 167 bp之间,手工校正后长度为1 137 bp,保守位点1 126个,变异位点11个,简约信息位点6个,碱基转换和颠换分别为3和2,比值为1.6;MP和NJ两种方法所得结果几乎一致,均将5种忍冬属药材分为两大支,黄褐毛忍冬与忍冬为第一大支,红腺忍冬、华南忍冬和灰毡毛忍冬为第二大支。结论:rbcL基因能够从分子水平揭示5种忍冬属药材的差异,为忍冬属药材鉴定以及系统发育提供重要参考依据。
Objective： To identify different Lonicera in the South of Zhejiang province by ribulose 1, 5-bisphosphate carboxylase large （rbcL） gene analysis. Methods： The primers designed by Primer Premier 5.0 to amplify the rbcL. ClustalX sequenced the rbcL gene. MEGA computed the value of transitional pairs and transversional pairs. The system diagram of genetic relationship was constructed by Nelghbor-Joining and Maximun Parsimony, the out-group was Heptacodium miconioides. Bootstrap evaluated reliability of diagram. Results： 1 143 - 1 167 bp nucleotide were sequenced in rbcL from Lonlcra plant, and remaining 1 137 bp after revision, the conserved site, varible site and Parsim-Information site were 1 126 bp, 11 bp and 6 bp respectively. The value of the transitional pairs and transversional pairs was 3 and 2. The Si/Sv was 1.6. Both NJ and MP could divide Lonicera into two groups, one was the Lonlcera japonica and Lonlcera fulvotomentosa, the other was the Lonlcera macranthiodes, Lonicera hypoglauca and Lonicera confusa. Conclusion： The rbcl gene plays an important role in identifing the different Lonlcera plant.
出处
《温州医学院学报》
CAS
2012年第6期549-552,共4页
Journal of Wenzhou Medical College
