摘要
目的 探讨IL 1β、TNF α对成骨细胞增殖及c Fos、c Jun的影响。 方法 采用阶段性酶消化法分离培养新生大鼠颅盖骨成骨细胞 ,用MTT法观察成骨细胞增殖 ,用免疫组化法结合计算机图像处理系统检测成骨细胞c Fos、c Jun。结果 IL 1β和TNF α均能刺激成骨细胞增殖 ,此作用不被消炎痛所阻断。用IL 1β和TNF α刺激成骨细胞 30分钟时c Fos,c Jun即增加 ;c Fos在 6 0分钟 ,c Jun在 12 0分钟分别达到最高值 ,随后有所下降。结论 IL 1β和TNF α可以直接刺激大鼠颅盖骨成骨细胞增殖及诱导c Fos、c Jun增加。
Objective To determine the effects of IL 1β and TNF α on the proliferation and c Fos, c Jun of osteoblasts in vitro. Methods Osteoblast cells from rat calvaria were isolated by sequential enzymatic digestion. With 48 hours treatment of various concentration IL 1β and TNF α, the proliferation of osteoblasts was measured by tetrazolium salt (MTT) method, and c Fos and c Jun of osteoblasts with the treatment of IL 1β and TNF α for various durations were detected by the immunohistochemistry combined with computer based image analysis method. Results Both IL 1β and TNF_α were able to stimulate osteoblast proliferation. Osteoblasts were preincubated by indomethacin for 2 hours, and then treated with IL 1β (100U/ml) or TNF α(10U/ml) for 48 hours. The results showed that indomethacin could not block the osteoblast proliferation effect induced by IL 1β or TNF α. Meanwhile, c Fos and c Jun of osteoblasts increased at 30 min after the stimulation of IL 1β and TNF α, with a maximum at 60 min and 120 min respectively, and decreased afterward. Conclusion IL 1β and TNF α are able to directly stimulateratcalvariaosteoblastproliferation, and c Fos and c Jun of osteoblasts increase rapidly, which probablymediatedtheeffectof IL 1β and TNF_α on osteoblast proliferation.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2000年第1期42-45,共4页
Chinese Journal of Endocrinology and Metabolism
关键词
白细胞介素1
肿瘤坏死因子
成骨细胞
Interleukin 1
Tumor necrosis factor
Osteoblasts
Proto oncogene proteins c fos
Proto oncogene proteins c jun
