摘要
目的在动物水平探索口服柔嫩梭菌(Clostridium leptum)对哮喘小鼠气道炎症的影响。方法建立OVA致敏的BALB/c小鼠哮喘模型,依据检测气道炎症情况和气道反应性确定哮喘模型构建成功。30只BALB/c小鼠随机分为3组:正常对照组,安慰剂组和口服柔嫩梭菌治疗组,每组10只。通过HE染色检测小鼠肺组织病理变化,细胞计数检测肺泡灌洗液中炎性细胞(嗜酸性粒细胞、中性粒细胞、淋巴细胞、巨噬细胞)的数目,ELISA方法检测肺泡灌洗液中炎性因子(IL-4、IL-5、IL-13)的表达情况,并检测各组小鼠的气道反应性。结果验证OVA致敏哮喘小鼠模型构建成功;口服柔嫩梭菌可显著减轻OVA致敏小鼠的气道高反应性,气道炎症细胞浸润和炎性细胞因子分泌(P<0.05)。结论口服柔嫩梭菌可显著减轻OVA致敏小鼠的气道炎症和气道高反应性,这可能为哮喘治疗提供新思路。
Objective To investigate the influence of oral Clostridium leptum on the asthma mice. Methods OVAsensitized BALB/c mice model was established. Ten OVA-sensitized and Clostridium leptum （CL） -fed BALB/c mice were randomized into CL-treated group, while another twenty mice served as placebo group （ten mice） and blank group （ten mice） respectively. The pathological changes of the lung tissue were determined by HE staining; the numbers of macrophages, eosinophils, lymphocytes and neutrophils in bronchoalveolar lavage fluid （BALF） were counted; the Th2 cytokine were measured by ELISA, and the airway responsiveness was measured. Results The asthma mice model was successfully established. CL administration significantly decreased airway hyperresponsiveness, reduced the airway inflammatory cell infiltration and inflammatory cytokine secretion in OVA-sensitized mice, compare to placebo group （P 〈 0.05 ）. Conclusion Oral Clostridium leptum can significantly reduce airway inflammation and airway hyperresponsiveness in OVA-sensitized mice, which may provide new ideas for the treatment of asthma.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第11期966-968,972,共4页
Chinese Journal of Microecology
基金
国家自然科学基金资助项目(81200013)
关键词
柔嫩梭菌
气道炎症
哮喘
Clostridium leptum
Airway inflammation
Asthma
