摘要
目的:观察纯化后的星虫多糖(Sipunculus nudus polysaccharide,SNP)的抗疲劳作用。方法:以不同剂量的SNP(50,100,200 mg·kg-1)对小鼠ig给予受试物7 d后测定各组的小鼠力竭游泳的持续时间,运动后血清尿素氮(BUN)、肌糖原、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。结果:小鼠给药7d后,对照组负重游泳时间为(115±40)s,SNP中、高剂量组负重游泳时间分别为(206±93),(203±74)s,与对照组比较显著延长(P<0.01);力竭运动90 min后,对照组BUN含量为(8.72±0.46)mmol·L-1,SNP中、高剂量组分别为(7.55±0.70),(7.89±0.42)mmol·L-1,与对照组比较显著降低(P<0.01);对照组肌糖原含量为(0.41±0.04)mg·g-1,SNP中、高剂量组含量分别为(1.01±0.18),(1.06±0.22)mg·g-1,与对照组比较显著提高(P<0.01)。此外,SNP高剂量组肝组织中SOD,GSH-Px活性明显高于对照组(P<0.01)。结论:星虫多糖具有显著的抗疲劳作用,并有其最适剂量。
Objective： It was reported that Sipunculus nudus polysaccharide （SNP） had effects on fatigue in the early time in our laboratory, in the article we studied the anti-fatigue effects of purified SNP on the fatigue resistance. Method： The treatment groups were given various doses of SNP （50, 100,200 mg·kg^-1） for 7 consecutive days, respectively, and the longest swimming time was detected after loaded swimming test. the level of serum urea nitrogen （BUN）, muscle glycogen and the activity of hepatic antioxidase including superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were detected. Result： After 7 days, the mouse swimming time in the blank control group was （115 ±40） s, and the swimming time with medium and high dose feeding of purified Sipunculus nudus polysaccharide was （206 ± 93 ）, （ 203 ± 74） s, respectively, longer than blank control groups （P 〈 0.01）. The serum urea nitrogen of the treated groups was （8.72 ± 0.46） mmol ·L^-1, and that in medium and high dose group was （7.55 ± 0. 70）, （7.89 ± 0. 42） mmol·L^-1, respectively, lower than blank control groups （P 〈0.01）. Besides, the muscle glycogen in the blank control group was （0.41 ± 0. 04） mg ·g^-1, and that in medium and high dose groups was （ 1.01 ± 0. 18） , （ 1.06 ± 0. 22） mg ·g^-1 , higher than blank control groups （P 〈0.01）. Moreover, the activity of SOD and GSH-Px in high dose group in hepatic tissue was increased compared with blank control group （ P 〈 0.01 ）. Conclusion ： SNP has noticeable anti-fatigue effect on mice, especially in intermediate at dose group （100 mg ·kg^-1） and its strongest effect on most biomarkers is found when given an intermediate dose.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第23期212-215,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
星虫多糖
负重游泳
肌糖原
血清尿素氮
抗氧化酶
抗疲劳
Sipunculus nudus polysaccharide
loaded swimming test
muscle glycogen
serum urea nitrogen
hepatic antioxidase
anti-fatigue
