摘要
以鱼粉含量为25%的饲料为对照组,分别用酵母提取物替代配方中15%、30%、45%和60%鱼粉,配制5组等氮、等能饲料投喂凡纳滨对虾(Litopenaeus vannamei)(7.50±0.13)g 42d,研究酵母提取物替代鱼粉对凡纳滨对虾血清免疫酶及鳃组织中溶菌酶mRNA及Toll受体mRNA相对表达量的影响,并进行溶藻弧菌(Vibrio alginolyticus)人工急性感染试验。结果表明:15%组血清溶菌酶(LSZ)活性显著高于对照组(P<0.05),其余替代组与对照组无显著差异。酵母提取物部分替代鱼粉对凡纳滨对虾血清超氧化物歧化酶(SOD)活性和鳃组织中溶菌酶mRNA及Toll受体mRNA的相对表达量影响均不显著(P>0.05);经溶藻弧菌人工急性感染后,24h和48h时60%组累计死亡率显著高于对照组(P<0.05),其余各替代组与对照组差异不显著(P>0.05),72h和96h时各替代组与对照组差异均不显著(P>0.05)。表明以酵母提取物替代凡纳滨对虾饲料中45%的鱼粉不会显著降低凡纳滨对虾的免疫抗菌能力。
The basal diet（A） contains 25% fish meal as control,and four isonitrogenous isoenergic yeast extract experiment diets,which substituted 15%（B）,30%（C）,45%（D） and 60%（E） fish meal in basal diet,were fed to the Litopenaeus vannamei（7.50±0.13） g respectively for 42 d,then superoxide dismutase（SOD） activity and lysozyme（LSZ） activity in haemolymph and the expressions of lysozyme mRNA and Toll receptor mRNA in gill were detected.The shrimp were also challenged with Vibrio alginolyticus.The results showed that： LSZ activity in haemolymph in 15% group was significantly higher than that of control（P0.05）.There was no significant difference of SOD activity in haemolymph,the expressions of lysozyme mRNA and Toll Receptor mRNA in gill between alternative groups and control group（P0.05）.The cumulative mortality of 60% group at 24 h and 48 h was significant higher than that of control（P0.05）,and there was no significant difference of cumulative mortality between alternative groups and control group at 72 h and 96 h（P0.05）.It is therefore suggested that yeast extract can replace 45% fish meal of the diets without affecting immunity and vibrio-resistant ability of the shrimp.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2012年第6期1003-1010,共8页
Journal of Shanghai Ocean University
基金
上海市科学技术委员会项目(08DZ1981000)
上海市重点学科建设项目(Y1101)
