摘要
为了培育葡萄脱毒苗木,更进一步促进葡萄组织培养技术的研究,对影响葡萄离体快繁的若干因素进行试验,选出最适葡萄离体快繁的培养基。取不同状态(休眠期、生长期)的2年生枝条作为材料,消毒后在超净工作台上切成1.5cm带有腋芽的茎段进行培养对比,发现春季即将解除休眠的枝条上的冬芽发芽率和成活率最高、生长势最好。继代增殖培养基MS+6-BA 1.0mg/L+IBA 0.1mg/L的组合生长的有效苗最多,生根培养基为1/2MS+IBA 0.4mg/L,IBA最有利于葡萄的生根,这个浓度中的苗炼苗的成活率最高。在试验过程中最佳增殖组合中偶尔也会有玻璃苗出现,所以此次试验得到的最佳继代培养基只是在这几种浓度中的最好配比组合,应将浓度在现有的最佳配比范围上下浮动进行更进一步的研究,而对于生根培养也可尝试用1/4MS、1/6MS、1/8MS进行进一步的试验研究。
In order to cultivate grapes detoxification seedlings and further promote the study of grape tissue culture techniques, a number of factors affecting the vitro rapid propagation of grapes were tested and the culture medium most suitable for the grape in vitro rapid propagation was elected. 2-year-old branches at different states （dormant period, growing season） were taken as the material which was cut into 1.5 cm stem segments after disinfection in the ultra-clean work bench to be cultured to contrast. It was found that in spring, the winter buds of the branches after which were relieved dormancy had the highest sprout rate and survival rate and the best growth potential. The combination of growth of the culture medium of subculture multiplication MS＋6-BA 1.0 mg/L＋ IBA 0.1 mg/L had the maximum effective seedlings. The rooting culture medium, 1/2MS ＋ IBA 0.4 mg/L, IBA was the most conducive to the rooting of grape, the seeding in this concentration had the highest survival rate. In the process of the experiment, the vitrification seeds occasionally appeared in the optimum proliferation combination, therefore, the best subculture medium obtained in the experiment was just the best proportioning combination. A further study on that the concentration is adjusted plus or minus within the range of the best proportioning should be done. For rooting culture, 1/4MS, 1/6MS, 1/8MS try to be used for further nilot studv_
出处
《农学学报》
2012年第10期55-57,67,共4页
Journal of Agriculture
基金
新疆克拉玛依市科学技术局科研专项"设施栽培条件下无公害绿色精品农业种植技术与示范推广"(SK2004-03)
新疆克拉玛依市科学技术局科研专项"优良沙旱生植物引种栽培试验研究及沙地造林技术推广与示范"(SK2010-41)
关键词
葡萄脱毒苗木
离体快繁
有效苗
继代增殖培养基
生根培养基
Grapes Detoxification Seedlings
Vitro Rapid Propagation
Effective Seedlings
SubcultureProliferation Medium
Rooting Culture Medium
