摘要
选用8个核桃无性系作为试验材料,经不同低温(4℃、-3℃、-6℃、-9℃、-12℃,4℃为对照)处理后,测定其叶片电导率、可溶性糖和丙二醛(MDA)含量,对核桃抗寒性进行研究。结果表明:随着胁迫温度的下降,叶片相对电导率呈现出"S"型上升趋势,核桃离体叶片半致死温度(LT50)在-5～12℃之间;可溶性糖与丙二醛含量的变化为先增加再下降,均在-6℃出现峰值,但抗寒性与可溶性糖含量呈正相关,而与丙二醛含量呈负相关。3种方法测定的核桃抗寒性基本一致,表明3个指标均可作为核桃抗寒性的鉴定方法。
Cold resistance of 8 Juglans sigillata clones were examined by measuring the indices such as e- lectrical conductivity, soluble sugar and malondialdehyde （MDA） contents in the leaves that were underg- one different low temperatures （4℃ ,--3℃ ,--6℃ ,--9℃ ,--12℃ ,in which 4℃ was used as control）. The results showed that with the decrease of the stress temperature, the relative electrical conductivity tended to rise as a ＂S＂ curve. The semilethal temperatures of the leaves in vitro（LTs0）were between --5℃ to 12℃. Soluble sugar and MDA contents increased firstly then decreased with the decrease of temperature, two indices all arrived the maximum values at --6℃. The content of soluble sugar was positively correla- ted with the cold resistance, MDA however, was negatively correlated with the severeness of the coldness. The results of the cold resistance of 8 clones from the three evaluation indices were basically identical, in- dicating that they all could be applied in the evaluation of the cold resistance of J. sigillata.
出处
《西北林学院学报》
CSCD
北大核心
2012年第6期12-15,25,共5页
Journal of Northwest Forestry University
基金
云南省林业厅“核桃良种扩繁技术推广”科技推广项目(2009TK055)
关键词
核桃无性系
低温胁迫
抗寒性
生理指标
Juglans sigillata clone
low temperature stress
cold resistance
physiological index
