摘要
拉曼光谱具有快速、灵敏、无损、实时监测等显著特点,在微生物学领域得到广泛应用。分别介绍共焦显微拉曼光谱、共振拉曼光谱、表面增强拉曼光谱、拉曼成像、相干反斯托克斯拉曼光谱、激光镊子拉曼光谱和Raman-FISH的原理和特点,并重点总结和分析不同拉曼光谱技术在微生物的结构、化学组成,以及代谢过程等相关研究中的应用优势。合理利用这些技术在基础微生物、发酵微生物和微生物诊断等方面具有潜在的应用价值。
Raman spectroscopy has been applied extensively in the field of microbiology in recent years due to its characteristics of quickness,sensitivity,non-destructiveness and Real-time detection.In this paper,we first introduce the principles and characteristics of confocal Raman microspectroscopy,resonance Raman spectroscopy,surface-enhanced Raman,Raman imaging,coherent anti-Stokes Raman,laser tweezers Raman and Raman-FISH respectively,and then focus on the application advantages of different Raman spectroscopy technologies employed to study the structure,chemical composition and metabolism of microbes.The proper use of these technologies in basic microbiology,microbial fermentation,diagnostic microbiology and other related aspects is very potential,especially in application.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第10期63-68,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(31060128)
广西科学院基本科研业务费资助项目(11YJ24WL01)
广西师大博士科研启动基金
关键词
拉曼光谱
微生物
应用
Raman spectroscopy Microbe Application
