摘要
目的了解新生儿脐血NK细胞和CD56bright CD16dim/-NK细胞亚群的比率,探讨CD56bright CD16dim/-NK细胞在脐血低免疫应答中的作用。方法使用血细胞分析仪计数淋巴细胞;采用流式细胞术检测脐血、母亲及对照组中NK细胞和CD56bright CD16dim/-NK细胞亚群的比率。结果脐血中淋巴细胞计数显著高于母亲及对照组;NK细胞比率无显著性差异;CD56bright CD16dim/-NK细胞高于母亲及对照组;母亲组CD56bright CD16dim/-NK细胞高于对照组。结论脐血中丰富的CD56bright CD16dim/-NK细胞是脐血低免疫应答的原因之一。
Objective The proportions of NK cells and CD56 bright CD16dim/-subset NK cells among the umbilical cord blood of newborns and the peripheral blood from their mothers were analyzed to explore the role of CD56 bright CD16dim/-NK cells in hypo-immune response of umbilical cord blood.Methods Lymphocytes were counted by Sysmex XE2100 hematocyte cytometer;Flow Cytometry was used to determine the percentages of NK cells and CD56 bright CD16dim/-subset NK cells in the blood from umbilical cord and the peripheral blood from mothers and control groups.Results Compared to the mothers and control groups,the quantity of lymphocytes were significantly higher in the umbilical cord blood（P0.01）,However,no statistical differences were observed on the proportions of NK cells（P0.05）.The proportion of CD56 bright CD16dim/-subset NK cells in umbilical cord blood was higher than the proportion in the mothers group which displayed higher CD56 bright CD16dim/-subset cells than the cells in the control group.Conclusion The abundance of CD56 bright CD16dim/-subset NK cells in umbilical cord blood is one of the reasons for the hypo-immune response of umbilical cord blood.
出处
《中国实验诊断学》
2012年第11期2060-2062,共3页
Chinese Journal of Laboratory Diagnosis
基金
南京市医学发展重点项目(ZKX08030)
