摘要
运用免疫组织化学方法研究了褪黑激素受体亚型Mel1a在大弹涂鱼视网膜、脑部、垂体、松果体、性腺和小肠中的分布.结果表明,Mel1a分布于视网膜的感光细胞层、外丛状层、内核层和神经节细胞层;小脑分子层;视顶盖边缘层和视觉层;垂体的神经垂体和腺垂体的中外侧部;松果体终囊及背囊;精巢的间质细胞、精原细胞、精母细胞以及精子细胞;卵膜的滤泡细胞;小肠的柱状上皮细胞外沿和肌肉层.研究结果提示褪黑激素参与大弹涂鱼多种生理功能的调节,为进一步揭示Mel1a在硬骨鱼体内的分布及其作用提供了科学依据.
Melatonin and its receptors are the essential regulators that transmit environmental stimulations to the brain in the verte- brates,and may also regulate the bi-monthly spawning of the mudskipper （Boleophthalmus pectinirostris） during the spawning sea- son. However,the localization and tissue expression of melatonin receptors in mudskipper are lacking. In the present study,the ex- pression of melatonin receptor subtype Mell, in retina, brain, pituitary, pineal gland,gonad, small intestine of the mudskipper was ex amined using immunohistochemistry. We found immuno-positive reaction to Mella antibody in the photosensory cells, outer plexiform, internal nuclear and internal plexiform layers of the retina. The immuno-positive responses were also observed in the cerebellum,the optic tectum＇ s stratum fibrosum marginale, stratum marginal, neurohypophysis, proximal pars distalis of the pituitary, the pineal vesi- cle, dorsal sac of the pineal gland, leydig cells, spermatogonia, spermatocytes, spermatid, follicle layer of ovary, the outside edge of co- lumnar epithelium,and the muscular layer of the intestine. The extend expression of melatonin receptor, Mel_1a ,in the wide-range tis- sue types suggest that melatonin may be involved in the regulation of multiple physiological functions including the reproduction in the mudskipper. This study was a necessary step in the way to more precisely identify the sites of the melatonin receptor subtype Mel_1a in the fish,its regulation and respective roles.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第6期1092-1096,共5页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金项目(40976095)
福建省科技计划重点项目(2008N0041)
关键词
大弹涂鱼
褪黑激素受体
免疫组织化学
Boleophthalmus pectinirostris
melatonin receptor
immunohistochemistry
