摘要
将油松种子置于(25±1)℃培养箱中培养,通过分析胚乳内GA3、IAA、ZR及ABA含量的变化,探讨种子萌发初始阶段胚乳内源激素的作用机理。结果表明:油松种子胚乳内源激素在萌发初始阶段都有不同程度的变化。其中GA3含量在培养最初27h变化不明显,随后逐渐升高,在培养结束时达到最大值18.87ng/g。IAA和ZR的含量表现出先升高再降低的趋势。其中ZR含量在培养最初9h变化较小,随后快速升高,在培养45h达到最大值15.57ng/g后,又缓慢降低。IAA在置床最初18h含量基本保持在22.22～22.54ng/g,随后迅速升高,在培养54h达到峰值29.06ng/g后,含量开始降低。ABA含量在培养最初18h变化不明显,随后快速降低,在培养72h时降为最低值40.10ng/g。胚乳中GA3/ABA、IAA/ABA及ZR/ABA比值在置床初期变化较小,随后逐渐升高,在培养结束时达到最大值。油松种子胚乳内源激素含量的变化与萌发进程相一致。
The seeds of Pinus tabuliformis were cultivated at （25 ±1 ） ℃ and the mechanism of endogenous hormone of endosperm during initial germinating stage was discussed by measuring the changes of GA3 , IAA, ZR and ABA contents. The results showed that the contents of endogenous hormone changed differently during initial germinating stage. The content of GA3 did not change obviously during the first 27 hours of cultivation and increased afterwards to the peak value with 18.87 ng/g at 72nd hour. But those of IAA and ZR increased at first and then decreased. The content of ZR changed a little in the first 9 hours of cultivation and increased afterwards to the peak value with 15.57 ng/g at 45th hour,and then decreased. The IAA content during the first 18 hours was basically stable ranged from 22.22 to 22.54 ng/g and increased rapidly afterwards to the maximum value with 29.06 ng/g at 54th hour, and decreased afterwards. The content of ABA did not change greatly during the first 18 hours of cultivation and decreased rapidly afterwards to the lowest level at 72nd hour （40. 10 ng/g）. The ratios of GA/ABA,ZR/ ABA and IAA/ABA changed a little in the early stage and increased afterwards to the peak value at 72nd hour. The changes of endogenous hormone of endosperm were consistent with seed germination.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2012年第6期30-33,共4页
Journal of Beijing Forestry University
基金
教育部科技基础条件平台"林木和花卉种质资源标准化整理
整合与共享"(505002)
关键词
油松种子
萌发初始阶段
内源激素
Pinus tabuliformis seeds
initial germinating stage
endogenous hormone
