摘要
对中国对虾(Fenneropenaeus chinensis)能否产生类免疫保护作用进行了初步探讨。将健康的中国对虾接种经不同辐照强度γ射线灭活的白斑综合征病毒(white wpot wyndrome virus,WSSV),连续投喂接种20 d后,对其存活个体进行抗WSSV性能测试,检验其存活情况。结果显示,至216 h实验组(射线辐照剂量分别为5 kGy、10 kGy、15 kGy和20 kGy)存活率分别为45.5%、54.2%、68%和65.4%,存活对虾的病毒携带量分别为(14.0±11.9)、(14.1±4.3)、(24.5±15.0)和(15.1±7.8)copies/ng DNA,死亡对虾病毒携带量分别为(2.1±2.0)×105、(5.2±4.4)×105、(3.8±4.4)×105和(6.2±7.7)×105copies/ng DNA,阳性对照组存活率为20%,其存活和死亡对虾病毒携带量分别为(24.7±7.4)×105copies/ng和(5.8±6.9)×105copies/ng DNA。以上结果说明,中国对虾可能存在类免疫保护作用,而且通过接种经γ射线灭活的WSSV能够提高对虾后续抗感染能力。研究结果旨为采用免疫学手段预防对虾白斑综合征提供参考。
In order to explore the quasi-immune response of Fenneropenaeus chinensis,healthy shrimps were vac-cinated with inactivated white spot syndrome virus（WSSV）.WSSV was inactivated using varying doses of γ-ray irradiation.After 20 days of continuous feeding with inactivated WSSV,the surviving shrimps were exposed to active WSSV to test their survival rates.It was shown that over 216 hours,the survival rates attributable to IE-5 kGy,IE-10 kGy,IE-15 kGy and IE-20 kGy were 45.5%,54.2%,68%,and 65.4%,respectively;the mean WSSV loads of the surviving shrimps were（14.0±11.9）,（14.1±4.3）,（24.5±15.0） and（15.1±7.8） copies/ng DNA,com-pared with that of dead shrimps of（2.1±2.0）×105,（5.2±4.4）×105,（3.8±4.4）×105 and（6.2±7.7）×105 copies/ng DNA,respectively.Within the positive control group,the survival rate was 20%,and the mean WSSV copies of surviv-ing and dead shrimps were（24.7±7.4） and（5.8±6.9）×105 copies/ng DNA,respectively.It is suggested that the presence of a quasi-immune response system in F.chinensis is possible,and exposure to WSSV inactivated by γ-ray irradiation can improve the anti-virus resistance of the shrimps.These data show that WSSV can be pre-vented by vaccination,thereby providing data references for developing a healthy culture system for F.chinensis.
出处
《中国水产科学》
CAS
CSCD
北大核心
2012年第6期989-993,共5页
Journal of Fishery Sciences of China
基金
国家自然科学基金面上项目(31072206)
山东省自然科学基金青年基金项目(ZR2009DQ002)
中央级公益性科研院所基本科研业务费专项资金项目(20603022011010)
农业部948课题(2011-G12)
关键词
中国对虾
类免疫
Γ射线
白斑综合征病毒
Fenneropenaeus chinensis
quasi-immune response
γ-ray
white spot syndrome virus（WSSV）
