摘要
从活性污泥中筛选出一株高效反硝化菌aHD7,30℃静置培养3d,脱氮率可达91.7%,且反应过程中亚硝酸盐积累量较低,3d后亚硝酸盐氮浓度基本稳定在1.8mg·L-1.显微镜观察显示,菌株为革兰氏阴性杆菌,大小为0.5μm×(1.5～2.5)μm.通过生理生化特性及16SrDNA同源性分析,初步推断该菌株为门多萨假单胞菌(Pseudomonasmendocina).考察了碳源、C/N、氮初始浓度、pH等因素对菌株反硝化性能的影响.结果表明:对中低浓度硝酸盐(硝酸盐氮浓度≤276.95mg·L-1),脱氮率接近100%,硝酸盐氮浓度高达553.59mg·L-1时,脱氮率可达66.8%,且亚硝酸盐积累量甚微;最适碳源为乙醇;C/N为6～8和偏中性条件有利于反硝化反应.aHD7具有较强的厌氧氨氧化性,平均氨利用率达4.56mg·L-1·d-1.
A highly efficient denitrifying bacterium ariD7 was screened from activated sludge. After static culture at 30 ℃ for 3 days, the denitrification rate of the ariD7 reached 91.7% , and during denitrification, nitrite had lower accumulation, with its concentration basically maintained at 1.8 mg. L-1. The microscopy observation demonstrated that the ariD7 was a gram-negative bacillus, with an average size of 0.5 μm×（ 1.5-2.5 ） μm. Based on its biochemical/morphological charac- teristics and homologic analysis of 16S rDNA sequence, the ariD7 was identified as Pseudomonas mendocina. The investigation on the factors affecting the denitrification capacity of ariD7 showed that at the initial concentration of nitrate nitrogen being less than 276.95 mg . L-1 , the denitrifica- tion rate was almost 100%, and when the initial concentration of nitrate nitrogen was as high as 553.59 mg . L-1 , the denitrification rate could reach 66.8%, with little nitrite accumulated. Etha- nol was the most suitable carbon source. C/N ratio 6-8 and pH value 6-9 benefited the denitrifica- tion. The aHD7 had a good ability of anaerobic ammonium oxidation, and its average ammonium utilization rate reached 4.56 mg.L-1. d-1.
出处
《应用生态学报》
CAS
CSCD
北大核心
2012年第11期3096-3102,共7页
Chinese Journal of Applied Ecology
基金
国家水体污染控制与治理重大科技专项(2008ZX07101-006-08)
浙江省重大科技专项(2009C03006-3)
温州招投标项目(F-GB201107110148
Z100602217)资助
关键词
反硝化菌
筛选
脱氮率
厌氧氨氧化
denitrifying bacterium
screening
denitrification rate
anaerobic ammonium oxida-tion.
