摘要
目的 探讨在脊髓压迫伤时微管相关蛋白 (MAP) 1B的表达及其意义。方法 用免疫组织化学方法对持续压迫和压迫后减压的大鼠脊髓中MAP1B和乙酰胆碱转移酶 (ChAT)的表达进行观察。结果 受压迫减压后的脊髓前角MAP1B有一高表达过程 ,而持续压迫的脊髓无明显高表达 ;减压后的脊髓前角ChAT的表达有所恢复 ,而持续压迫的脊髓前角ChAT的表达则明显受抑制 ;从时相上看 ,减压脊髓MAP1B高表达出现略晚于ChAT的恢复 ;减压MAP1B高表达活跃期与后肢活动功能指数恢复活跃期较为吻合。结论 受压脊髓减压后可有一神经轴索生长修复过程 ,在减压后第 10～ 30d内最为活跃。
Objective\ To investigate the expression of microtubule associated protein 1B (MAP1B) and choline acetyltransferase (ChAT) in spinal cord compressiion as well the implication. Methods\ The expression of MAP1B and ChAT in ventral horn of spinal cord following spinal cord compression or following decompression was observed by using immunohistochemical method. Results\ Following decompression of spinal cord an increased expression of MAP1B could be observed in the ventral horn, while could not following continuous compression of the cord. The expression of ChAT in ventral horn could be restored following decompression of spinal cord, but not following continuous compression of the cord. The increased expression of MAP1B was later than recovery of ChAT expression. There was a coincidence of upregulation of MAP1B expression with recovery of hindlimb function index. Conclusion\ There is neurite regeneration in ventral horn of spinal cord following decompression of compressed spinal cord, which is more active within 10 to 30 days following decompression of spinal cord.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第2期135-137,共3页
Chinese Journal of Experimental Surgery
关键词
脊髓压迫症
微管相关蛋白1B
CHAT
大鼠
Spinal cord
\ Spinal cord compression
\ microtubule associated protein 1B(MAP1B)
\ Choline acetyltransferase(ChAT)
