摘要
目的 :进一步探索淋系分化抗原在急性髓性白血病 ( AML )中的表达特征及其对 AML患者预后的影响。方法 :采用间接免疫荧光法对 84例初诊 AML 患者进行了免疫表型检测 ,并用流式细胞仪对淋、髓两系分化抗原共表者做双标记分析。结果 :84例 AML 中 ,3 0例 ( 3 5 .72 % )有淋系抗原表达 ( ly+ )。对于双表型患者 ,流式细胞仪分析显示其白血病细胞表型不均一。在同等化疗强度基础上 ,ly+ 者较 ly- 者完全缓解 ( CR1 )率低 ,获 CR1 所需时间长 ,CR1 后无病生存期短 ( DFS期 ,P值匀 <0 .0 5 )。多因素分析表明 ly+是影响 AML 患者 DFS期的一个主要危险因素 ( P<0 .0 5 )。结论 :淋系分化抗原在 AML 中的表达具有复杂性 ,ly+的 AML 属于预后不良的临床亚型 。
Objective:To further explore the expression of lymphoid associated antigens in acute myloid leukemia (AML) and its impact on clinical pragnosis of patients with AML. Methods:Using indirect immunofluorescence assay, immunophenotyping was performed in 84 patients with de nove AML. Leukemic cells coexpressing lymphoid and myeloid associated antigens were analysed by dual color flow cytometry. Thirty in 84 patients expressed lymphoid lineage markers (Ly + AML, 35.72%). Results from analysis of flow cytometry showed that leukemic cells in biphenotypic patients may be composed of dual marked cells, or dual and single marked cells. On the basis of the same chemotherapic intensity, Ly + AML patients ( n =30) had a lower complete remissin (CR) rate (81.48%v60%, P <0 05), longer time to achieve CR 1 (Median day: 29v24, P <0 05), short disease free survival (DFS) time (Median follow up week: 37, χ 2=4 52, P <0 05) than Ly - patients significantly. Multivariation analysis including age, sex, WBC count, days to achieve CR 1 and Ly +/- indicated that expression of lymphoid associated antigens in AML patients was a significant adverse factor to DFS time ( P <0 05). Conclusion:Expression of lymphoid associated antigens in AML showed complexity. Ly + AML is a clinical subtype which has a poor prognosis. Therapeutic design direct to it remains to be further explored.
出处
《军医进修学院学报》
CAS
2000年第1期29-31,共3页
Academic Journal of Pla Postgraduate Medical School
关键词
淋系分化抗原
急性髓性白血病
预后
双表型
lymphoid associated antigens
AML
prognosis
biphenotype
flow cytometry
