摘要
目的 比较观察超极化停搏与高钾停搏的心肌保护效果 ,并评价低温对心脏超极化停搏的影响。方法 18条犬分为三组 ,每组 6条。低温高钾组、常温超极化组和低温超极化组 ,分别以4℃标准St.Thomas液 (K+ 16mmol/L)、含吡那地尔 5 0 μmol/L的 37℃St.Thomas液 (K+ 5mmol/L)和含吡那地尔 5 0 μmol/L的 4℃St.Thomas液 (K+ 5mmol/L)为心停搏液。阻断循环共 6 0min ,恢复循环后观察 2 0min。测定心肌过氧化脂质、腺苷酸含量和血清心肌酶活性指标。结果 低温高钾组在阻断 6 0min和开放 2 0min ,心肌各项指标均显示有严重的缺血和再灌注损害 ;而超极化组损害较轻 ,特别是低温超极化组 ,损害轻微。结论 用ATP敏感性钾通道开放剂吡那地尔诱导的心脏超极化停搏 ,其心肌保护的效果明显优于传统的高钾组 。
Objective To evaluate the myocardial protective effect of hyperpolarized heart arrest at different temperature during cardiopulmonary bypass CPB.Methods Eighteen dogs were randomly allocated to be infused with St.Thomas solution containing 50μmol/L pinacidil and 5mmol/L K + at 37℃ (group A) or 4℃ (group B), or the standard St.Thomas solution containing K + 16mmol/L at 4℃ (group C) respectively, through aortic root after aortic clamping . The global surgical ischemia lasted 60min with the declamping of 20min .The activities of serum myocardial enzymes, and levels of lipid peroxide and adenine nucleotide of myocardium were measured.Results All paramaters showed to occur serious ischemic and reperfusion damages during the whole procedures in group C, while there were the mild damages in two hyperpolarized groups, especially in group B.Conclusions Myocardial protective effect of hyperpolarized arrest induced with pinacidil, an ATP sensitive potassium channel opener, is superior to that of traditional hyperkalemic cardioplegia , which is much more prominent in hypothermic state.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第5期301-304,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金!资助项目 3976 0 0 71
关键词
心脏停搏
心麻痹液
心肌再灌注损伤
吡那地尔
Heart arrest,induced
Cardioplegic solutions
Myocardial reperfusion injury
Pinacidil
