摘要
【目的】为了探讨电阻抗参数是否能够监测梨叶片受胁迫程度,【方法】以鸭梨(Pyrus bretschneideri‘Yali’)为试材,0.2 mmol·L-1水杨酸诱导后接种轮纹病菌,研究梨叶片电阻抗参数及与膜脂过氧化相关的生理参数的变化。【结果】结果表明,水杨酸处理后叶片的胞外电阻率增大,接种轮纹病菌叶片的胞外电阻率、胞内电阻率和弛豫时间分布系数减小,而弛豫时间增大。水杨酸诱导后超氧化物歧化酶(SOD)活性增强,丙二醛(MDA)和超越阴离子(O.2)含量降低。对电阻抗参数与生理参数的相关性分析表明,接种病菌叶片的胞内电阻率变化与MDA含量显著正相关,水杨酸诱导后叶片的胞内电阻率、胞外电阻率的变化与O.2含量显著正相关,而弛豫时间则均与叶片中O.2含量为显著负相关。【结论】电阻抗参数能够反映梨叶片细胞内的变化,可以作为监测梨叶片受胁迫程度的物理指标。
【Objective】 The study is to investigate whether parameters of electrical impedance spectroscopy（EIS） could be used to monitor the stress level in pear leaves.【Method】In this study,0.2 mmol·L-1 salicylic acid（SA） was applied and pear（Pyrus bretschneideri ＇Yali＇） leaves were as test materials.After the treatment with SA,pear leaves were inoculated with Physalospora piricola Nose.The effects of SA and pathogen stress on parameters of EIS in pear leaves were investigated.The changes of physiological parameters related with membrane lipid peroxidation were also examined.The correlations between EIS parameters and physiological parameters were analyzed.【Result】The results showed that specific extracellular resistance in leaves treated with SA increased.It was also observed that specific extracellular resistance,specific intracellular resistance and distribution coefficient of relaxation time decreased,but relaxation time increased in leaves infected by P.piricola.In the inoculated leaves,the SA-treatment enhanced the activities of superoxide dismutase（SOD） and the contents of MDA and ■ dropped down simultaneously.These results demonstrated that SA could reduce the degree of membrane lipid peroxidation.Correlation analysis between EIS parameters and physiological parameters in pear leaves showed that intracellular resistance in leaves infected by P.piricola was positively correlated with contents of MDA.Furthermore,specific extracellular resistance and specific intracellular resistance were positively correlated with contents of ■ in leaves treated with SA,but relaxation time was negatively correlated with contents of ■.【Conclusion】EIS parameters which reflected the changes within cells could be used as index of monitoring the pathogen stress in pear leaves.
出处
《果树学报》
CAS
CSCD
北大核心
2012年第6期1031-1035,共5页
Journal of Fruit Science
关键词
梨
电阻抗参数
水杨酸
轮纹病菌
Pear
Electrical impedance parameter
Salicylic acid
Physalospora piricola Nose
