摘要
目的 了解扩张后皮肤慢性损害的动态修复过程和近、远期皮肤回缩机理。方法 在犬皮肤扩张后不同时段取材 ,分别用电镜观察皮肤结构的变化 ,用免疫组化方法观察真皮层α 平滑肌肌动蛋白 (α SMA)的表达情况 ,用Fura 2钙探针检测真皮成纤维细胞中钙离子浓度的变化 ,同时采集扩张皮肤中的成纤维细胞通过体外三维培养观察细胞密度和胶原浓度对成纤维细胞收缩率的影响。结果 扩张后真皮α SMA染色阳性的成纤维细胞量少散在 ,而成纤维细胞中钙离子浓度在扩张后显著升高。成纤维细胞收缩率与其密度呈正相关而与胶原浓度呈负相关。结论 扩张后皮肤的修复是介于完全再生和瘢痕修复之间的中间态过程。增生的功能活跃的成纤维细胞是扩张皮肤回缩的动力 ,而胶原的溶解、变性 。
Objective\ The aim of this study is to observe the repairing process of skin chronic injury due to expansion and explore the mechanism of skin contracture after expansion.Methods\ 20 dogs used in this study were divided randomly into four groups.Skin samples were collected from the back of dogs when expansion finished and 3 months,6 months, 1 year after expansion.Normal skim from the other side on the back of dogs was used as control group.The dermal ultrastructure was observed by electriscope.The expression of α SMA in dermis at different stage has been analyzed by immunohistochemistry,and the concentrationof intracellular free Ca 2+ in the fibroblast has been measured by Fura\|2 fluorescen indicator.Meanwhile we constructed a 3\|dimension system in vitro to analyze the afgfect of different cell density and collagen concentration to fibroblast contracture.Results\ (1)The microfilment in fibroblast was fragile,and the features of myofibroblast was absent,the collagen fibers were returned to reticular arrangement along with time.One after expansion few of the collagen remained irregular in arrangement and aparse.(2)After expansion the concentration of Ca 2+ increased significantly.(3)In 3\|D culture system the density of fibroblasts positively corresponded to fibroblast contracture and the collagen concentration negatively corresponded to it.Conclusion\ After expansion the dog skin has a structure between scar tissue and thoroughly regeneration skin.Dermal fibroblast is the dynamic source of the skin contracture,meanwhile the density of fibroblast and collagen concentratrion are two essential factors during skin contracture\;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2000年第3期143-145,共3页
Chinese Journal of Plastic Surgery
基金
国家自然科学基金青年基金资助
