摘要
目的：从基因转录水平探讨中药天癸方对雄激素致不孕大鼠（ASR）肥胖及无排卵的影响。方法：采用α－32P标记瘦素（leptin）受体（OB－R）及神经肽Y（NPY）寡核苷酸探针原位杂交、放射自显影技术结合图像分析，观察中药治疗前后下丘脑弓状核（AR）两种物质mRNA的表达变化。结果：ASR中ARC的NPY基因表达水平明显增加（P＜0．01），OB－R基因表达水平明显降低（P＜0．01），ASR呈肥胖、无排卵状态。用中药天癸方治疗后，NPY基因表达下降，OB－R基因表达上升至正常大鼠水平。结论：ASR肥胖及无排卵可能与NPYmRNA过度表达、OB－RmRNA数目异常有关，中药可调节NPY和OB－RmRNA表达而发挥减肥及促排卵作用。
To explore the effect of Tiangui Recipe (TGR) on obesity and anovulation in androgen-sterilized rats (ASR) from the gene transcription level. Methods: In situs hybridization and autoradiography were adopted, using α-32 P-radiolabelled leptin receptor (OB-R) and neuropeptide Y (NPY) oligonucleotides probes and integrating with image pattern analysis, to observe the effect of TGR on changes of OB-R, NPY mRNA expression in the hypothalamic arcuate nucleus (ARC). Results: Compared to the normal rats, the levels of hypothalamic OB-R mRNA expression decreased, NPY mRNA expression elevated significantly in ASR (P < 0.01 ).ASR characterized by obesity and anovulation. There was no significant difference in hypothalamic OB-R, NPY mRNA expressions between normal rats and ASR with TGR administration. Conclusion: The increased hypothalamic NPY and decreased OB-R mRNA expression may be important contributing factors to the development of obesity and anovulation in ASR. TGR may play a role in reducing weight and inducing ovulation by regulating NPY/OB-R mRNA expression.
出处
《中国中西医结合杂志》
CSCD
北大核心
2000年第5期362-364,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
上海市医学领先专业重点学科中西医结合妇科资助!96001
关键词
天癸主
不育症
LEPTIN受体
MRNA
原位杂交
Tiangui Recipe
androgen-sterilized rat
neuropeptide Y
leptin receptor
messenger ribonucleic acid
in situs hybridization
