摘要
为了解环境理化因子作用对机体产生的生物效应 ,采用3 H TdR掺入法研究了甲基汞和不同剂量的电离辐射对小鼠胸腺DNA合成及适应性反应的影响。结果表明 :甲基汞可以抑制胸腺细胞DNA合成 ;低剂量的电离辐射 ( 0 0 75Gy)对胸腺DNA合成具有一定刺激作用 ,3 H TdR掺入量明显高于对照组 ;而高剂量的电离辐射 ( 2Gy)对胸腺细胞DNA合成有抑制作用 ;经低剂量电离辐射预处理后再进行甲基汞染毒时比单独用甲基汞染毒组的损伤减轻 ,3 H TdR掺入量增加 ;先给予高剂量电离辐射再进行甲基汞染毒比单独用甲基汞染毒组胸腺细胞3 H TdR掺入量明显下降。提示低剂量电离辐射可以产生兴奋刺激作用 ,对DNA损伤产生保护作用。其机理有待于进一步研究。
In order to understand the biological effects of environmental chemical and physical factors on the organism,the influence of methtylmercury and ionizing radiation of different dose levels on adaptation and DNA sythesis of thymus of mice was studied by means of 3H TdR incorporation method.The results showed that meththylmercury could inhibit DNA synthesis of thymocytes.Low dose radiation(0 075 Gy)could stimulate DNA synthesis of thymocytes yhe amount of 3H incorporation was higher than that of control.High dose radiation(2Gy) could inhibit DNA synthesis of thymocytes.The degree of DNA damagewas obviously decreased and amount of 3 H incorporation was increased in LDR(0 075Gy)pretreatment group.The degree of this effect in HDR(2Gy)plus Me Hg was significantly lower than MeHg.It indicated that LDR could induce Hormesis,and protect from DNA damage.The relative mechanism could be further discussed.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第5期393-394,共2页
Chinese Journal of Public Health
基金
国家自然科学基金
