摘要
目的 研究人工关节中聚乙烯微粒诱发骨溶解的原因 ,探讨微粒物质介导骨溶解的机理。方法 建立一个聚乙烯微粒诱发骨溶解的兔动物模型 ,观察聚乙烯微粒诱发骨溶解的组织学改变。通过体外培养人外周血单核细胞对聚乙烯微粒反应 ,检测培养上清液中肿瘤坏死因子 (TNF-α)含量。结果 聚乙烯微粒引起假体周围一层纤维结缔组织界膜形成 ,界膜中可见大量巨噬细胞、异物巨细胞和聚乙烯微粒。体外研究发现 ,聚乙烯微粒可刺激单核细胞产生更多的 TNF- α。结论 聚乙烯微粒可以引起假体周围的骨溶解 ,从而导致假体松动。聚乙烯微粒可以激活外周血单核巨噬细胞 ,使其释放更多的
Objective To study the etiology and the mechanisms of osteolysis induced by polyethylene particles Methods A rabbit model of resorption of bone at the bone prosthesis interface induced by polyethylene particles was established The pathological findings were studied In vitro human monocytes were cultured and stimulated by polyethylene particles The levels of TNF α of the supernats were measured by radioimmunoassay Results There was a layer of interface membrane that contained numbers of macrophages and giant cells around the prosthesis, and there was evidence of active bone resorption and osteolysis The polyethylene particles were found in the interfacemembrane and engulfed by macrophages The levels of TNF α were significantly increased when the monocytes were exposed to polyethylene particles ( P <0 01) Conclusion There is a positive relationship between the accumulation of polyethylene particles and subsequent resorption of bone The polyethylene particles can stimulate monocytes to produce more TNF α
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
2000年第3期209-211,共3页
Journal of Nanjing Medical University(Natural Sciences)
关键词
人工关节
聚乙烯微粒
骨溶解
实验研究
prosthesis
osteolysis
polyethylene particles
animal experiment
