摘要
目的探讨乳腺癌干细胞MDR1表达与乳腺癌不同分子分型的关系。方法根据ER、PR、Her-2及CK5/14的水平划分为5个分子亚型,分为5组,通过PCR法分析不同分子分型乳腺癌组织中癌干细胞MDR1表达,分析乳癌干细胞中MDR1表达量与乳腺癌不同分子分型的关系。结果 Lumina1 A型组癌干细胞MDR1量(0.26±0.04)和Lumina1 B型组(0.31±0.03)最少,两组MDR1量无显著性差异(P>0.05);HER-2^+型组乳腺癌干细胞MDR1量(0.56±0.05)明显多于A组和B组,具有显著性差异(P<0.05);Basal-like型癌干细胞的MDR1量(0.98±0.01)和Normal-like型的MDR1量(0.90±0.15)最多,两组无显著性差异(P>0.05),但明显多于Luminal A型/B组、HER-2(+)型组,具有显著性差异(P<0.05)。结论乳腺癌干细胞MDR1表达与其分子分型有关。
Objective To investigate the association between MDR1 gene expression in breast cancer stem cells and the molecular subtypes of breast cancer tissue. Methods According to ER, PR, Her-2 and CK5/14 expression profiles, 153 breast cancer specimens were divided into 5 molecular molecular subtypes, in which the expression of MDR1 was detected to analyze the relationship between MDR1 gene expression and the subtypes of breast cancer stem cells. Results The expression of MDR1 in Luminal A subtype breast cancer was 0.26±0.04, which showed no significant difference from that of Luminal B subtype （0.31±0.03, P〉0.05）. Compared with these two subtypes, HER-2 （＋） subtype breast cancer tissues showed a significantly higher MDR1 expression（0.56±0.05, P〈0.05）. MDR1 expression in Basal-like subtype and Normal-like subtype breast cancers was comparable （0.98±0.01 vs 0.90±0.15, P〉0.05）, but both significantly higher than that in Luminal A and B subtypes and HER-2 （＋） subtype （P〈0.05）. Conclusion The expression of MDR1 gene in cancer stem ceils is related with the molecular subtypes of breast cancer tissue.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2012年第11期1636-1638,共3页
Journal of Southern Medical University
基金
福建省卫生厅青年基金(2010K737)
关键词
乳腺癌
癌干细胞
多药耐药
分子分型
breast cancer
stem cancer cell
MDR1
molecular subtypes
