摘要
目的探讨大鼠腮腺主导管结扎后细胞程序性死亡分子5（programmed celldeath5，PDCD5）的表达及其与腮腺细胞凋亡的关系，以期了解PDCD5在腮腺萎缩中的作用。方法对66只Wistar大鼠建立腮腺主导管结扎动物模型，分别在结扎0、1、3、5、7、14、21、30、60、90和120d（每个时间点6只大鼠）获取腮腺标本，免疫组化法检测腮腺细胞中PDCD5的表达，脱氧核糖核酸末端转移酶介导的原位缺口末端标记法检测腮腺细胞的凋亡。结果PDCD5蛋白在正常的腺泡细胞和导管细胞中呈低水平表达，均匀分布于胞质。腮腺主导管结扎后，PDCD5蛋白的表达水平显著上调，在结扎后3d腺泡细胞中PDCD5的吸光度值达峰值（1．261±0．048），胞质和胞核中均有表达。在结扎后1、3d，腺泡细胞中PDCD5的吸光度值均显著高于导管细胞，二者差异有统计学意义（P〈0．01）。细胞凋亡检测结果显示，结扎后3d腮腺细胞凋亡指数显著增高，腺泡细胞凋亡指数[（21．750±0．119）％]显著高于导管细胞[（5．720±0．205）％]，二者差异有统计学意义（P〈0．01）。PDCD5蛋白的表达水平与腮腺主导管结扎诱导的细胞凋亡呈显著正相关（r=1，P=0．003）。结论大鼠腮腺主导管结扎后PDCD5的表达与腮腺萎缩过程密切相关，PDCD5在腮腺结扎后的细胞凋亡途径中可能发挥重要作用。
Objective To investigate the expression and relationship of programmed cell death 5 （PDCD5） and cell apoptosis in the parotid gland after leading duct ligation in rat and elucidate the role of PDCD5 on the atophy of parotid glad. Methods The Wistar rat model of leading duct ligation was established, and the samples of parotid gland were obtained from different time point （0, 1, 3, 5, 7, 14, 21, 30, 60, 90 and 120 d）. The expression of PDCD5 protein was examined by immunohistochemistry. Cell apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL）. Results The distribution of PDCD5 protein in normal parotid was in cytoplasm with uniformity. The expression of PDCD5 protein was significantly increased and reached the peak at 3 d（ 1. 261±0. 048 ） following main duct ligation. PDCD5 was located both in cytoplasm and nuclear of parotid gland cells. The PDCD5 density in acinar cells was higher than that in duct cells at day 1 and 3 after duct ligation（P 〈0. 01 ）. The apoptotic ceils were obviously upregulated at 3 d after duct ]igation. The apoptosis index observed in acinar cells[（21.750 ±0. 119）%] was more than that in duct cells[（5.720±0.205）%]. The difference of apoptosis index between acinar cells and duct cells was statistically significant （ P 〈 0. 01 ）. The increased PDCD5 levels were positively correlated with cell apoptosis induced by duct ligation. Conclusions The expression of PDCD5 is associated with the atophy of the parotid glad after rat parotid duct ligation, indicatinz that PDCD5 might olav an imoortant role in aDontotic pathwavs after Darotid duct libation.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2012年第11期694-697,共4页
Chinese Journal of Stomatology
基金
基金项目：国家自然科学基金（30871263）
山东省医药卫生科技发展计划（2011HW004）
