摘要
目的探讨T淋巴瘤侵袭转移诱导因子1（Tiaml）蛋白与乳腺癌侵袭转移之间的关系及其相关机制。方法采用免疫组织化学法检测126例乳腺癌组织标本中Tiaml的表达情况。应用RNA干扰（RNAi）技术沉默Tiaml，检测磷酸化细胞外调节蛋白激酶1（ERKI）、ERK2和血管内皮生长因子（VEGF）表达的变化及转录因子AP-1转录活性的变化。结果Tiaml在乳腺癌组织中的染色主要定位于细胞质，呈棕黄色，且Tiaml在淋巴结转移的乳腺癌组织中的表达明显高于无淋巴结转移的乳腺癌组织（P〈0．05）。应用特异性siRNA沉默Tiaml后，乳腺癌细胞中磷酸化ERKl、ERK2和VEGF蛋白的表达明显下调，转录因子AP-1的转录活性降低。结论Tiaml在乳腺癌侵袭转移过程中具有重要作用，Tiaml可能是通过ERK、AP-1和VEGF通路影响其血管生成。
Objective To investigate the significance of Tiaml in invasion and metastasis of breast carcinoma and its mechanisms. Methods Immunohistochemistry was used to detect Tiara1 expression in tumor tissue of 126 breast carcinomas. Tiaml was silenced by siRNA in breast carcinoma cell line MDA-MB- 435, then the expressions of phosphor-ERK 1, ERK 2 and VEGF were detected, and electrophoretic mobility shift assay （EMSA） was used to examine the transcription activiy of AP-1. Results There was a significant relationship between Tiaml expression and lymph node metastasis （ P 〈 0. 05 ）. Furthermore, after silencing of Tiaml, the exp^sions of phosphor-ERK 1, ERK 2 and VEGF were decreased, and the transcription activity of AP-1 was down-regulated in the MDA-MB-435 cells. Conclusion Tiaml is closely related with invasion and metastasis of breast carcinoma, and the cascade Tiaml through ERK, AP-1 and VEGF pathways may play an important role in enhancing angiogenesis, therefore, to promote invasion and metastasis of breast carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2012年第11期831-834,共4页
Chinese Journal of Oncology
