摘要
[目的]应用响应面法优化食尼古丁节杆菌发酵产海藻糖合成酶的工艺。[方法]首先以培养温度、初始pH、摇床转速、接种量、发酵时间为因素进行单因素试验,在此基础上,应用Box-Behnken中心组合设计,以摇床转速、接种量和发酵时间为因素,以食尼古丁节杆菌发酵产海藻糖合成酶酶活的变化为响应值进行试验,运用SAS统计软件对试验数据进行分析,建立二次响应面回归模型,得出重要因素的最佳水平,从而确定最佳的发酵产酶条件。[结果]经响应面法优化获得食尼古丁节杆菌发酵产海藻糖合成酶的工艺参数为转速172.60 r/min、接种量13.96%、发酵时间55.97 h,在此优化条件下,海藻糖合成酶酶活力达到645.48 U/g。[结论]该研究可为海藻糖的生产提供科学依据。
[Objective] To optimize the process parameters of producing trehalose synthase by Arthrobacter nicotinovorus fermentation.[Method] The influence of fermentation temperature,initial pH,rotational speed,inoculation amount and fermentation time on trehalose synthase enzyme activity producing by Arthrobacter nicotinovorus fermentation were studied through single factor experiment,and approximately determined the amount of the various factors.Then the rotational speed,the inoculation amount and the fermentation time were selected as three factors,the center combination of Box-Behnken design experiments was used to study the change of trehalose synthase enzyme activity in the fermentation as response value.The experiment dates were analyzed by the SAS statistical software to set up quadratic response surface regression model for obtaining the the optimal level of the important factor,determining the optimum conditions of fermentation.[Result] The response surface method results showed that the optimum condition of producing trehalose synthase by Arthrobacter nicotinovorus fermentation was as follow： the rotational speed 172.60 r/min,the inoculation amount 13.96% and the fermentation time 55.97 h,in the optima conditions,the trehalose synthase enzyme activity was 645.48 U/g.[Conclusion] The study can provide scientific basis for improving the enzyme activity of trehalose synthase produced by Arthrobacter nicotinovorus fermentation.
出处
《安徽农业科学》
CAS
2012年第32期15873-15876,共4页
Journal of Anhui Agricultural Sciences
基金
农业科技成果转化资金项目(2010GB2C500236)
关键词
海藻糖合成酶
海藻糖
食尼古丁节杆菌
发酵
响应面法
Trehalose synthetase
Trehalose
Arthrobacter nicotinovorus
Fermentation
Response surface methodology
