摘要
目的初步探讨表达IL-4受体的调节性T细胞（IL-4R＋Treg）在过敏性哮喘中的临床意义。方法本研究纳入对屋尘螨过敏的20例哮喘患者作为研究对象，行肺功能检查和哮喘控制问卷（asthmacontrolquestionnaire，ACQ）调查，20名健康志愿者作为对照组。采用流式细胞术检测外周血IL-4R＋Treg／Treg百分比。结果间歇轻度组（n=12）、中重度组（n=8）和正常对照组（n=20）IL-4R＋Treg／Treg％分别为（4．24±2．14）％、（14．56±4．19）％和（1．04±0．54）％，各组之间差异有统计学意义（Pd0．001）。哮喘组IL-4R＋Treg／Treg％与FEV-％预计值呈显著负相关性（r=-0．512，P〈0．05），与ACQ得分呈显著正相关性（r=0．592，P〈0．01）。结论Treg表达的IL-4R在过敏性哮喘的免疫反应中可能发挥重要作用，IL-4R＋ Treg细胞数量的增多可导致哮喘患者体内免疫失衡而促进哮喘的发生发展。
Objective To investigate the clinical significance of regulatory T cells with IL-4R expression （IL-4R＋ Treg） in allergic asthma. Methods 20 patients with chronic persistent asthma were enrolled, lung function was tested and asthma control questionnaire （ACQ） was recorded. 20 healthy donors were enrolled as control. The proportions of IL-4R＋ Treg in peripheral blood were determined by flow cytometry. Results The percentages of IL-4R＋ Treg/Treg in peripheral blood with intermittent-mild and moderate-severe asthma were higher than that in healthy controls [（4.24±2.14）% vs （1.04± 0.54） %, P 〈0. 001] ;[（14.56±4.19） % vs （1.04±0.54）%, P 〈0. 001], and intermittent-mild asthma was significantly lower than moderate-severe asthma [（4.24± 2.14）% vs （14.56±4.19）%, P 〈 0. 001]. The percentage of IL-4R＋ Treg/Treg in peripheral blood with asthma was negatively associated with FEV1 pred （r =-0. 512, P 〈0.05）, and positively associated with the score of ACQ（ r = 0. 592, P 〈 0.01）. Conclusions We speculated IL-4R expressed by Treg play an important role in patients with asthma on the immune response, the increased number of IL-4R＋ Treg resulted in immune response imbalances and promote the occurrence and progress of asthma.
出处
《国际呼吸杂志》
2012年第20期1525-1528,共4页
International Journal of Respiration
基金
上海市自然科学基金基础重点项目（项目编号：08JC1415200）
