摘要
利用纤维素酶辅助提取茶籽粕中的茶皂苷。以茶皂苷得率为指标,通过单因素试验和正交设计优化提取工艺。最佳提取工艺参数为:pH 5.1,酶解温度47℃,酶解时间130 min,纤维素酶加入量1.125 mg/mL。在此条件下茶皂苷得率为20.23%。对纤维素酶酶解前后的脱脂茶籽粕进行的扫描电镜分析表明:酶解后茶籽粕颗粒表面变得粗糙、颗粒之间相互粘连,证实了纤维素酶对茶籽细胞壁的破坏作用。
The cellulase - assisted extraction of tea saponin from tea seed meal was studied. The extraction was optimized by single factor experiment and orthogonal design with the yield of tea saponin as an indicator. The optimal extraction parameters were as follows： pH 5.1, enzymolysis temperature 47 ℃, enzymolysis time 130 min, dosage of cellulase 1. 125 mg/mL. Under the optimal conditions, the yield of tea saponin achieved 20.23%. The SEM of the defatted tea seed meal before and after enzymolysis showed that the surface of the tea seed meal after enzymolysis turned to be rough and adhered to each other, which demonstrated that the cell wall of tea seed was destroyed by cellulase.
出处
《中国油脂》
CAS
CSCD
北大核心
2012年第11期54-57,共4页
China Oils and Fats
基金
陕西省重点实验室项目
陕西省科技厅科技攻关项目(2010K01-083)
云南民族大学民族药资源化学国家民族事务委员会-教育部重点实验室项目
关键词
纤维素酶
茶皂苷
提取
正交设计
cellulase
tea saponin
extraction
orthogonal design
