摘要
为研究含硫芳香族化合物的生物降解途径 ,采用静息细胞技术提取假单胞杆菌 ( Pseudomonassp.)不同部位的酶 .结果表明 ,对硝基苯砜基乙酸异丁酯降解酶主要定域在细胞膜周及膜内 .被试化合物在膜内酶的作用下 ,发生碳硫键断裂 ,降解产物是对硝基苯磺酸 .在膜周酶的作用下 ,发生碳硫键和酯的烷氧键 2种方式的断裂 ,生成对硝基苯磺酸和对硝基苯砜基乙酸 .
In order to study the biodegradation way of an aromatic sulfurcontaining compound,the resting cell technique was used to get different enzyme.The experimental results show that the degradation enzyme for 4NO2PhSO2CH2COOC4H9i mainly locates in cell and on the membrane.The enzyme in cells converts the tested compound to 4NO2PhSO3H with bond cleavage between carbon and sulfur.By the enzyme located on the membrane the compound is degraded as 4NO2PhSO3H and 4NO2PhSO2CH2COOH.The later is yielded with the bond cleavage between carbonyl and alkoxy group.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2000年第4期90-93,共4页
Environmental Science
基金
国家自然科学基金!资助项目 ( 2 98771 0 2 )
关键词
降解酶的定域
胞内产物
含硫芳香族化合物
鉴定
pnitrophenylsulfonyl isobutyl acetate
resting cell technique
localization of degradation enzyme
products in cells
