摘要
目的:研究红归胶囊对人鼻咽癌细胞菌株CNE2的抑制和诱导凋亡的干预作用,并揭示其机制。方法:采用MTT、TUNEL、流式细胞术(FCM)、ELISA和实时荧光定量PCR(qPCR)法检测不同浓度红归胶囊对人鼻咽癌细胞CNE2的生长、凋亡、细胞周期及对血管内皮生长因子(VEGF)和环氧化酶-2(COX-2)蛋白和基因表达影响。结果:红归胶囊作用后CNE2细胞抑制率明显增加,且呈浓度及时间依赖性,以浓度100μmol·L-1时效果最好,96h抑制率为80.35%,与空白组比较有统计学意义(p<0.01);有明显诱导凋亡的作用(p<0.01);用药后细胞周期G0/G1期,S期,G2/M细胞增殖分别受到抑制;COX-2、VEGF蛋白和COX-2mRNA、VEGFmRNA的表达减少。结论:红归胶囊具有抑制鼻咽癌细胞增殖和诱导细胞凋亡的作用,其抗癌机制可能与抑制COX-2、VEGF的表达有关。
Objective： To evaluate the surpressing and inducing apoptosis effect of Honggui capsule on human nasopharyngeal carcinoma cell line CNE2, and to analyze the possible mechanism. Methods： We used MTT, TUNEL, Flow cytometry （FCM）, ELISA and Real-time quantitative （qPCR） to measure the inhibition rate, apoptosis ratio, cell cycle and the expression of COX-2 and VEGF protein and mRNA of different concentration-rations of Honggui capsule on CNE2. Results： It was shown that the inhibition rate of CNE2 cell was significantly increased by Honggui capsule in concentration and time-dependent manners. The peak value （80.35%） reached at 100 ~a mol/L Honggui capsule for 96h, and the inhibition rate was significant differences compared to control group （ P 〈0.01）. Honggui capsule could also induce apoptosis （ p 〈0.01）. The cell cycle was arrested at G0/G1, S, G2/M. The results of EISA and RT-PCR showed that Honggui capsule reduce the expression of COX-2 and VEGF. Conclusion ： Honggui capsule can inhibit the proliferation CNE2 cells and induce apoptosis, and its mechanism maybe relate with inhibiting the expression of COX-2 and VEGF.
出处
《中国药物评价》
2012年第1期21-24,共4页
Chinese Journal of Drug Evaluation
