摘要
以商业栽培的25个香菇(Lentinula edodes)品种为材料,应用SSR分子标记技术进行区别性分析。本研究使用14对引物,引物的多态性为100%,每对引物产生的等位基因数为2～9个,平均5.0个,基因型数为2～12个,平均6.3个。预期杂合度为0.1151～0.8131,平均预期杂合度为0.6126;PIC值为0.1064～0.7736,平均PIC值为0.5541。25个品种中,除申香10号和申香12号不能区分外,对其他23个品种清晰鉴别,为构建香菇栽培品种的SSR分子指纹图谱提供了依据和方法。本方法获得的数据可以成为重复性良好、实验室间可比对的香菇栽培品种标准指纹图谱,在品种特异性鉴定中不再需要已有所有品种做参照,较RAPD、ISSR、SRAP等鉴定方法工作量大大减少。
The distinctness of 25 commercial cuhivars was identified with SSR marker from 14 pairs of primers for Lentinula edodes. The polymorphism was 100% for all of primers. The 2-9 alleles were generated for one primer pair and the 5.0 alleles was average to each primer pair. The 2-12 genotypes were formed for one primer pair and the 6.3 genotypes was average to each primer pair. The expect heterozygosity of 0. 1151-0. 8131 was presented and with average of 0. 6216. The PIC values ranged from 0. 1064 to 0. 7736 and with average of 0. 5541. The 23 varie- ties were separated clearly from each other by the 14 primer pairs except the one cluster consisted of Shenxiangl0 and Shenxiangl2. This result provided the basic technique for making SSR fingerprint profile to identify Lentinula edodes cuhivars. The SSR profile is suitable to construct standard fingerprint for identification of the cuhivars and it is well repeated and easily contrasted with various operators and labs compared with the profiles from RAPD, ISSR, and SRAP. The operator no longer puts all of the presented cultivars as reference to be tested in the identification so that the workload reduced greatly.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2012年第6期1067-1072,共6页
Journal of Plant Genetic Resources
基金
作物种质资源保护项目(NB2010-2130135-37)
