摘要
目的观察碱性成纤维细胞生长因子（bFGF）对大鼠骨髓间充质干细胞（BMSCs）体外增殖及其分化潜能的影响。方法体外培养大鼠BMSCs，分别设对照组和bFGF处理组，bFGF作用浓度分别为1、10、100μg／L。作用72h后，噻唑蓝（MTT）测吸光度（A）值反映细胞增殖。细胞免疫组织化学测细胞CIM4的累积A值；逆转录-聚合酶链反应（RT-PCR）检测细胞CIM4的mRNA相对表达量。结果在1μg／LbFGF作用下其MTTA值为0．334±0．036较对照组A值0．251±0．033明显增高（P〈0．05）。在1μg／LbFGF作用下其细胞免疫组织化学测得CD44的累积A值较对照组明显增高（P〈0．01）；其RT-PCR测得CIM4的mRNA相对表达量较对照组明显增高（P〈0．01）。结论作用浓度为1μg／L的bFGF可促进BMSCs的体外扩增并有助于保持BMSCs的分化潜能。
Objective This study was carried out to observe the effect of basic fibroblast growth factor （bFGF） on rat bone marrow mesenchymal stem cells （BMSCs） and its potentials of differentiation in vitro. Methods Rat BMSCs were cultured with a medium treated with bFGF at concentrations of 0, 1, 10, 100 μg/L. After 72 h of treatment, the effect of bFGF on BMSCs viability was tested using methyl thi- azol tetrazolium （MTT） assay for detection of optical density. The expressions of CD44 of BMSCs were in- vestigated by immunoeytochemical analysis and reverse transcription-polymerase chain reaction （RT-PCR） assay. Results After treatment of bFGF at concentration of 1 μg/L, the optical density （0. 334 ±0. 036） in 1 μg/L bFGF group was markedly increased compared with control （0. 251 ± 0. 033 ） （P 〈 0. 05 ）. The integrated optical density of C1M4 in 1 μg/L bFGF group was significantly increased, compared with con- trol （P 〈 0. 01 ）. The mRNA expression of CD44 in 1μg/L bFGF group was significantly increased, compared with control （P 〈 0. 01 ）. Conclusion After treatment of bFGF at concentration of 1 μg/L, bFGF can induce the proliferation of BMSCs and preserve its potential of differentiation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第11期2274-2276,共3页
Chinese Journal of Experimental Surgery
关键词
骨髓间充质干细胞
成纤维细胞生长因子
分化潜能
Bone marrow mesenchymal stem cells
Basic fibroblast growth factor
Potential of differentiation
