摘要
在近几年试验研究的基础上，提出冬春季温室切段扦插快繁马铃薯脱毒苗模式。试验结果表明，温室温度25～28℃，相对湿度90％，光照5000～10000lx，脱毒苗切段长2．5～3cm，蘸生根液NAA20mg/L＋KT2mg/L＋GA30．5m∥L，扦插密度1000。1300株，m。，扦插后喷雾生长调节液（NAA5mg/L＋6-BA0．5mg/L＋GA31mg/L）和1／2MS营养液或1，2K5营养液＋植物叶面肥，20—35d后，苗高8～10cm、茎节6—7个时，进行继代切段繁殖，如此重复继代切段繁殖3-4次，脱毒苗增殖20～25倍。
On the basis of the previous experiment results in recent years, the article studied the rapid propagation system of virus-free potato plantlets by cutting euttage in greenhouse in winter and spring. The results showed that the appropriate conditions for cutting cuttage and rapid propagation of potato virus-free plantlets were as follows： the temperature was 25- 28℃, with relative humidity of 90%, light intensity of 5000-10 000lx in greenhouse, cuttings length of 2.5-3 era, density of 1 000-1 300 euttings/m2, and the rooting medium was NAA 20 Ing/L＋KT 2 mg/L ＋GA3 0.5 mg/L. The cutting seedlings, sprayed with the growth regulator solution （NAA 5 mg/L＋6-BA 0.5 mg/L＋GA3 1 rag/L） and 1/2 MS nutrient solution or 1/2 K5 nutrient solution＋foliage fertilizer （Huashuo 828）, took roots and germinated very well. After 20-35 days of growth, the seedlings with height of 8-10 cm and 6-7 nodes were used as basic seedlings for the following subculture cutting euttage and breeding. Finally, the virus-free plantlets proliferated 20-25 times through 3-4 subculture cycles of cutting cuttage propagation.
出处
《长江蔬菜》
2012年第20期18-20,共3页
Journal of Changjiang Vegetables
基金
甘肃省农业生物技术研究与应用开发项目(2008-11)
陇原青年创新人才扶持计划项目(2008)
甘肃省农业科学院农业科技创新专项(2009GAAS18)
关键词
马铃薯
切段扦插
快繁
脱毒苗
Potato
Cutting cuttage
Rapid propagation
Virus-free plantlets
