摘要
[ Objective] The aim was to compare the application of two bioassays to monitoring and evaluation of water quality. [ Method ] By using two bioassays （micronucleus detecting technology based on Vicia faba root tip cells and the bioassay by luminous bacteria）, we monitored and evaluated seven kinds of single solutions respectively added different pollutants （ Hg, Cd, As, Cr6 ＋ , Pb, I_A8 and CODc,） and a mixed solution added those seven pollutants. Afterwards, we compared their results under the same pollutant and concentration, so as to study the two bioassays＇ sensitivity and sensitive concentration to the seven pollutants. [ Result] Under the same pollutant and concentration, micronucleus detecting tech- nology based on Vicia faba root tip cells had responses to Hg, Cd, As and Cr6＋ , but there was no response to Pb, LAS and CODer. However, the bioassay by luminous bacteria had responses to most pollutants except Cr6＋. Comparing the sensitive degree and concentration to each pollutant, they can complement each other. For these seven pollutants, the bioassay by luminous bacteria was better than micronucleus detecting technology based on Vicia faba root tip cells. Meanwhile, from the testing result of the mixed solution, the combined toxicity of several pollutants in lower con- cantrations was serious. At the same time, contrasted to normal chemical methods, bioassays were fast and effective. [ Conclusion] The research could provide theoretical references for the correlation study of bioassays.
[ Objective] The aim was to compare the application of two bioassays to monitoring and evaluation of water quality. [ Method ] By using two bioassays （micronucleus detecting technology based on Vicia faba root tip cells and the bioassay by luminous bacteria）, we monitored and evaluated seven kinds of single solutions respectively added different pollutants （ Hg, Cd, As, Cr6 ＋ , Pb, I_A8 and CODc,） and a mixed solution added those seven pollutants. Afterwards, we compared their results under the same pollutant and concentration, so as to study the two bioassays＇ sensitivity and sensitive concentration to the seven pollutants. [ Result] Under the same pollutant and concentration, micronucleus detecting tech- nology based on Vicia faba root tip cells had responses to Hg, Cd, As and Cr6＋ , but there was no response to Pb, LAS and CODer. However, the bioassay by luminous bacteria had responses to most pollutants except Cr6＋. Comparing the sensitive degree and concentration to each pollutant, they can complement each other. For these seven pollutants, the bioassay by luminous bacteria was better than micronucleus detecting technology based on Vicia faba root tip cells. Meanwhile, from the testing result of the mixed solution, the combined toxicity of several pollutants in lower con- cantrations was serious. At the same time, contrasted to normal chemical methods, bioassays were fast and effective. [ Conclusion] The research could provide theoretical references for the correlation study of bioassays.
基金
Supported by Scientific and Technological Planning Project of Tianjin City (05ZHGCNC01600)
Special Scientific Research Fee of Central Public Welfare Institutes (2009-XYP-06)
