摘要
【目的】调查黑龙江省大豆灰斑病菌(Cercospora sojina)的生理小种类型,分析黑龙江省大豆灰斑病菌各生理小种间的亲缘关系。【方法】2006—2011年,对黑龙江省进行大豆灰斑病样本的采集和病菌的分离。利用11对SSR引物对黑龙江省的24个灰斑病菌菌株进行EST-SSR基因型分析。【结果】共分离获得大豆灰斑病菌2 674株,鉴定出1—15号共15个生理小种。SSR结果共检测出等位变异46个,平均每个位点为4.2个,相似系数范围为0.091—0.956,平均相似性系数达到0.589。聚类分析结果与大豆灰斑病菌小种类型呈现出较高的相关性。【结论】黑龙江省大豆灰斑病菌间具有丰富的遗传多样性。大豆灰斑病菌的亲缘关系与生理小种类型密切相关,与采集年份、采集地点相关性不大。
【Objective】The objective of this study is to survey race types of Cercospora sojina and to analyze the genetic relationship of races of C. sojina in Heilongjiang Province. 【Method】Samples of C. sojina were collected and pathogens were isolated in Heilongjiang Province from 2006 to 2011. Twenty-four strains of C. sojina were analyzed by EST-SSR with 11 pairs SSR primers. 【Result】Fifteen races were identified from 2 674 strains of C. sojina obtained in this study. The results of SSR showed that the amount of the allelic variation in all strains was 46, and the average was 4.2. Similarity index between strains ranged from 0.091 to 0.956, and the average was 0.589. The cluster results had the high relativity to race of C. sojina strains.【Conclusion】There was a rich genetic diversity among C. sojina in Heilongjiang Province. The genetic relationship of C. sojina is closely related to race type, and is irrelevant to collective time and place.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第21期4377-4387,共11页
Scientia Agricultura Sinica
基金
黑龙江省自然科学基金项目(C200936)
国家公益性行业(农业)科研专项(201103016-03A2)
