摘要
目的:观察消痰通腑方对小鼠结肠癌CT26盲肠原位移植瘤肝转移的影响及肝组织中胰岛素生长因子IGF-I/IR表达的影响,探讨消痰通腑方干预结肠癌肝转移的作用机制。方法:将造模成功的小鼠随机分为模型组、中药组,另设正常组,每组10只,中药组给予消痰通腑方灌胃,每日2次。待模型组小鼠成恶病质状态时,处死小鼠,取瘤组织及肝组织,观察各组荷瘤鼠肿瘤生长情况及抑瘤率;并运用免疫组化及实时荧光定量聚合酶链式反应(real time PCR)观察各组荷瘤鼠结肠癌组织IGF-I/IGF-IR蛋白及mRNA表达。结果:中药组小鼠瘤重、肝重、肝脏指数均低于模型组(P<0.05),与空白组比较,差异无统计学意义(P>0.05),中药组小鼠肝组织中IGF-I/IGF-IR蛋白及mRNA的表达均低于模型组(P<0.05),与空白组比较,差异无统计学意义(P>0.05)。结论:消痰通腑方可明显抑制小鼠结肠癌CT26原位移植瘤的生长,且能抑制肝脏转移,下调结肠癌肝转移组织中IGF-I/IGF-IR蛋白及mRNA的表达,可能是其抗结肠癌肝转移的机制之一。
Objective ： To observe the effects of Xiaotan Tongfu Recipe on IGF - I/IGF - IR protein and gene expres- sion in liver tissue of mice with tumor - burdened colon cancer and discuss Xiaotan Tongfu Recipe~ mechanism of action on colon liver metastasis. Methods ： The successful model of the mice were randomly divided into model group and Xiaotan Tongfu recipe group, normal group was set. Xiaotan Tongfu recipe was given to mice for 4 weeks in Xiaotan Tongfu recipe group. At the end of the experiment, the mice were killed and the mice＇s tumor weights, tumor suppression rates, liver weights,liver indexes, IGF - I/IGF - IR of the liver were detected by immunohistochemistry and real - time fluorescence quota PCR. Results ： The tumor weight,liver weight and liver index of Xiaotan Tongfu recipe group were significantly lower than that of the model group, compared with the model group,it was significantly diferent（ P 〈 0.05 ）, compared with the normal group,it had no significant difference（P 〉0.05 ）. IGF- I/IGF- IR protein and gene gexpression of model group was lower than that of model group, it was signifcantly diferent （P 〈 0.05 ）, compared with the normal group, it had no sig- nificant difference（ P 〉 0. 05 ）. Conclusion ： Xiaotan Tongfu Recipe could obviously inhibit the tumor weight of tumor - bear- ing mice, down - regulate the protein and gene expression of IGF - I/IGF - IR in liver tissue of tissue of mice with tumor - burdened colon cancer, and which may be one of the mechanism of Xiaotan Tongfu Recipe anti colon liver metastasis.
出处
《中华中医药学刊》
CAS
2012年第11期2557-2559,I0010,共4页
Chinese Archives of Traditional Chinese Medicine
