摘要
目的探讨高糖对人视网膜色素上皮（RPE）细胞表达血管内皮生长因子（VEGF）及色素上皮源性生长因子（PEDF）的干预作用。方法采用HRPE细胞株，将细胞分为正常对照组（NG，5．6mmol／L葡萄糖）、高糖组（HG，15、20、30mmol／L葡萄糖）、高渗组（HM，24．4mmol／L甘露醇＋5．6mmol/L葡萄糖）。应用逆转录PCR（RT—PCR）技术检测VEGF及PEDF mRNA的表达。用ELISA技术检测细胞上清液中VEGF蛋白的表达。结果高糖作用下VEGF mRNA和蛋白表达显著增高，PED FmRNA的表达受到显著抑制。结论高糖可从转录水平及蛋白水平诱导HRPE细胞VEGF的表达，并抑制PEDF的表达。
Objective To study the impact of high glucose concentration on the expression of vascular endothelial growth factor （VEGF） and pigment epithelium derived factor （PEDF） in cultured human retinal pigment epithelial ceils. Methods Human retinal pigment epithelial cells were divided into 5 groups according to the concentration of glucose in the culture media： normal glucose group （NG, 5.6 mmol/L）; high glucose group （HG1, 15 mmol/L D-glucose）; high glucose group （HG2, 20 mmol/L D-glucose）; high glucose group （HG3, 30 mmol/L D-glucose）; high osmolality group NG （HM, 24.4 mmol/L mannitol ＋）. The expression of VEGF and PEDF mRNA was detected by RT-PCR and the amount of VEGF in the HRPE-conditioned supematants was analyzed by enzyme linked immumosorbent assay （ELISA）. Results The difference of VEGF mRNA and VEGF protein between normal glucose group and that of high glucose group was statistically significant （P 〈0.05）. The difference of PEDF mRNA between normal glucose group and that of high glucose group was statistically significant （P 〈0.05）. Conclusions High glucose conditions induce the expression of VEGF mRNA, protein and suppress PEDF mRNA in cultured human retinal pigment epithelial cells.
出处
《中国实用眼科杂志》
CSCD
北大核心
2012年第11期1373-1376,共4页
Chinese Journal of Practical Ophthalmology
关键词
人视网膜色素上皮细胞
高浓度葡萄糖
VEGF
PEDF
Human retinal pigment epithelial cells
High glucose
Vascular endothelial growthfactors
Pigment epithelium derived factor
