摘要
目的探讨EphB4和EphrinB2的蛋白在视网膜母细胞瘤中的表达及其意义。方法应用免疫组织化学SP法测定10例正常视网膜组织和40例视网膜母细胞瘤标本中EphB4和EphrinB2的表达水平，分析其与性别、临床分期、分化程度和视神经浸润的关系。结果（1）EphB4和EphrinB2蛋白表达的阳性率，在正常视网膜组织中分别为5％和2．5％，40例视网膜母细胞瘤标本中分别为77．5％和67．5％。EphB4和EphrinB2在视网膜母细胞瘤组织中的阳性率与显著高于正常视网膜组织中的阳性率（P〈0．05）。（2）EphB4和EphrinB2的蛋白在视网膜母细胞瘤的阳性表达率与肿瘤组织的临床分期、分化程度及视神经浸润显著相关（P〈0．05）。（3）EphB4和EphrinB2的蛋白在视网膜母细胞瘤组织中的阳性表达显著相关（P〈0．05）。结论EphB4和EphrinB2可能在视网膜母细胞瘤的发生和发展过程中起重要作用，可作为评价视网膜母细胞瘤的浸润及预后的标志物。
Objective To investigate the protein expressions of EphB4 and EphrinB2 in retinoblastoma and to study their significance. Methods The expression of EphB4 and EphrinB2 was examined immunohistochemistrically by SP method in tissues of 10 normal retina and 40 patients with retinoblastoma and to study their relationship with gender, clinical stage, differentiation and optic nerve infiltration. Results 1.The protein expression of EphB4 and EphrinB2 observed in the normal tissues of retina was 5% and 2.5% respectively. In the 40 retinoblastoma cases, the positive rate of them was 77.5% and 67.5% respectively. The positive rate of EphB4 and EphrinB2 in retinoblastoma was higher than that in the normal retina （P 〈0.05）. 2.The positive expression of EphB4 and EphrinB2 protein were associated with clinical stage, differentiation and optic nerve infiltration （P 〈 0.05）. 3.The positive expression of EphB4 and EphrinB2 protein in retinoblastoma was positively correlated （P 〈0.05）. Conclusions EphB4 and EphrinB2 play important roles in carcinogenesis, development procedures of retinoblastoma and might be served as objective indicators of invasion and prog- nosis of retinoblastoma.
出处
《中国实用眼科杂志》
CSCD
北大核心
2012年第11期1321-1324,共4页
Chinese Journal of Practical Ophthalmology
