摘要
目的:探讨β-catenin在子宫内膜异位症(EMs)患者在位内膜的增殖与凋亡活性的关联,及其在EMs发病中的作用。方法:共收集68例EMs患者在位内膜组织(EMs组)和70例非EMs患者子宫内膜(对照组),采用免疫组织化学技术分析子宫内膜组织β-catenin的表达情况。TUNEL检测分析子宫内膜组织的凋亡活性,Ki67免疫组织化学染色分析子宫内膜组织的增殖活性。结果:与对照组比,EMs患者在位内膜Ki67表达明显增强,TUNEL阳性细胞明显减少(P<0.05)。在位内膜腺上皮及间质β-catenin表达明显高于对照组(P<0.05)。β-catenin在胞质及胞核的表达明显高于对照组(P<0.05)。直线相关分析结果显示无论是正常内膜还是EMs患者在位内膜,β-catenin的表达与Ki67表达均呈正相关,与TUNEL结果均呈负相关。结论:EMs患者在位内膜β-catenin的异常高表达可能通过上调内膜组织的增殖活性,下调其凋亡性而参与EMs的发病。
Objective： To investigate β-catenin expression and the apoptosis and proliferation activity in eutopic endometrium of women with or without endometriosis. Methods： The study was performed on 68 eutopic endometrium of patients with endometriosis and 70 normal endometrium of women without endometriosis. Immu- nohistochemistry was analyzed for Ki67 and β-catenin. Transferase-mediated dUTP nick-end labeling （TUNEL） assay was used to analyze apoptosis activity in endometrium tissue. Results： The study revealed a significantly elevated proliferation and reduced apoptosis in the eutopic endometrium of women with endometriosis compared with that of women without endometriosis. Compared with endometrium of women without endometriosis, glandu- lar and stromal β-catenin expression was significantly higher in eutopic endometrium of women with endometriosis. （P〈0.05）. For β-catenin immunoreactivity, there was a significant increase in cytoplasmic and nuclear β-catenin expression in eutopic endometrium of women with endometriosis than that of women without endometdosis （P〈0.01）. Conclusion： These findings suggest that abnormally high levels of β-catenin activity may be involved in the pathogcnesis of endometriosis, possibly by stimulating endometrial cell proliferation and depressing endomerial cell apoptosis.
出处
《生殖与避孕》
CAS
CSCD
2012年第11期733-738,759,共7页
Reproduction and Contraception
基金
上海市卫生局青年科研基金(2010Y015)
国家重大科学研究计划项目(2006CB944007)
上海市重点学科建设项目(B117)资助
