摘要
目的:观察慢性饮酒大鼠的肺组织及基质金属蛋白酶抑制剂(TIMP)-1等指标的变化,探讨乙醇与肺纤维化之间的关系及可能的机制。方法:健康雄性SPF级Sprague-Dawley大鼠20只,随机分成2组,对照组10只,每日给予定量无乙醇液体饲料单笼喂养;乙醇组10只,每日给予定量乙醇液体饲料单笼喂养,不再提供饮水。16周后,处死大鼠,并获取其肺组织,通过Masson染色观察肺间质中胶原沉积的情况,用比色法计算大鼠肺组织中谷胱甘肽(GSH)和羟脯氨酸(HYP)的含量,通过ELISA方法检测肺组织TIMP-1的含量。结果:乙醇组较对照组在肺泡间隔中胶原纤维沉积增多。与对照组比较,乙醇组肺组织GSH含量减少,HYP和TIMP-1含量升高,差异均有统计学意义(P<0.05或P<0.01)。结论:慢性摄入乙醇可以引起大鼠肺泡间隔胶原纤维沉积,肺组织中TIMP-1升高。饮酒可能是引起大鼠肺纤维化的原因之一。
Objective： To investigate the effect of chronic alcohol intake on pulmonary fibrosis and matrix metalloproteinase inhibitors （TIMP）-1 in rats,and their relationship and possible mechanism thereof. Methods： Twenty clean healthy male Sprague-Dawley rats were randomly divided into control group fed non-alcohol containing Lieber- DeCarli diet （n=10） and alcohol group fed alcohol containing Lieber-DeCarli diet （n=10）. Rats were killed after 16 weeks to get lung tissues. Masson staining collagen deposition was observed in alveolar interval. Contents of glutathione （GSH） and hydroxyproline（HYP） were measured by colorimetric. The level of TIMP-1 in lung tissues was detected by ELISA. Results： The more deposition of collagen fibers was found in alveolar septa of alcohol group than that of control group. Compared to control group, the content of GSH in rat lung tissues reduced significantly but HYP and TIMP-1 increased significantly in alcohol group （P 〈 0.05）. Conclusion： Chronic alcohol ingestion can cause collagen deposition in alveolar interval and higher expression of TIMP-1 in lung tissues. Alcohol may be one of reasons for pulmonary fibrosis in rats.
出处
《天津医药》
CAS
北大核心
2012年第11期1135-1137,共3页
Tianjin Medical Journal
基金
天津市卫生局科技基金资助项目(项目编号:2011kz39)
