摘要
目的探讨Nogo基因对心脏成纤维细胞增殖的调控作用,以及川芎嗪干预心肌成纤维细胞增殖的机理。方法培养新生大鼠心肌成纤维细胞,以实时荧光定量PCR和Western blot方法检测血管紧张素Ⅱ和不同浓度川芎嗪对培养心肌成纤维细胞Nogo表达和胶原合成的影响。结果在川芎嗪干预后,由血管紧张素Ⅱ诱导的Nogo-A mRNA、胶原ⅠmRNA表达升高出现了下降(P<0.05),而Nogo-B mRNA的表达下降在川芎嗪干预后有所恢复,与对照组相比,Nogo-A蛋白和Nogo-B蛋白亦出现类似变化。结论血管紧张素Ⅱ诱导大鼠心肌成纤维细胞胶原蛋白合成增加,而川芎嗪对其具有抑制作用,川芎嗪的抗纤维化作用可能与Nogo基因的调控作用有关。
Objective To investigate the effects of different doses of Tetramethylpyrazine （TMP） on collagen synthesis and Nogo expression in cardiac fibroblasts and the potential role of Nogo in myocardial fibrosis. Methods The cultured neonatal rat cardiac fibroblasts were induced by angiotensin Ⅱ and TMP at the same time. The mRNA expression of Nogo-A, Nogo-B and collagen Ⅱ were detected with real-time fluorescence quantitative PCR. And Nogo protein was semi-quantitatively detected by using Western blot method. Results Under the intervention of TMP, the increased mRNA expression of Nogo-A and collagen I induced by angiotensin Ⅱ were decreased, while the decreased mRNA expression of Nogo-B was increased. Compared with the control group, the expression of Nogo-A protein and Nogo-B protein showed similar changes as evaluated with Western blot. Conlusion TMP could inhibit collagen synthesis in cardiac fibroblast induced by angiotensin Ⅱ. The accompanying differential expression of Nogo-A and Nogo-B indicated that the anti-fibrosis effect of TMP might be related to the regulation effects of Nogo gene.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2012年第6期843-846,共4页
Journal of Sichuan University(Medical Sciences)
