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亲环素A保护PC12细胞对抗β-淀粉样蛋白诱导的氧化应激损伤和凋亡

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摘要 目的探讨亲环素A(CyPA)对Aβ25～35诱导PC12细胞氧化应激损伤和凋亡的影响。方法用不同浓度的CyPA预处理PC12细胞,再加入Aβ25～35继续培养,采用MTT法分析细胞存活率。用10 nmol/LCyPA预处理PC12细胞,再加入Aβ25～35继续培养,碘化丙啶(PI)单染后流式细胞仪进行凋亡的定量检测;罗丹明123(Rd 123)染色流式细胞仪检测线粒体跨膜电位;二氯荧光黄双乙酸盐(DCFH-DA)染色,倒置荧光显微镜和流式细胞仪检测细胞内活性氧(ROS)含量。结果 CyPA可提高细胞的存活率,减少Aβ25～35引起的细胞凋亡,CyPA还可以改善由Aβ25～35引起的线粒体跨膜电位的下降,降低Aβ25～35诱导产生的大量ROS。结论 CyPA可对抗Aβ25～35对PC12细胞的毒性作用,并通过增加线粒体跨膜电位和降低ROS产生从而减少细胞凋亡。
作者 葛宇松 尹琳
机构地区 大连医科大学附属第二医院神经内科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2012年第21期4722-4725,共4页 Chinese Journal of Gerontology
关键词 亲环素A AΒ25~35 凋亡 氧化应激
分类号 R749 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献9

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二级参考文献12

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中国老年学杂志
2012年 第21期

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