摘要
目的探讨洛铂对肝癌细胞的放疗增敏效应。方法用MTT法确定洛铂对肝癌细胞株HepG2半数抑制浓度(IC50),流式细胞仪检测1/4IC50、1/8IC50洛铂对HepG2细胞周期、凋亡的影响,蛋白免疫印迹检测凋亡相关蛋白Bcl-2、Bax、Caspase-3的表达,细胞克隆形成实验研究洛铂对HepG2的放射增敏比。结果洛铂对肝癌细胞株HepG2细胞毒作用呈剂量依赖性,半数抑制浓度为1.85μg/ml,不同浓度洛铂组(1/4IC50组、1/8IC50组)均观察到HepG2细胞凋亡和G2/M期阻滞明显增加(P<0.05);两组洛铂干预组肝癌细胞Bcl-2表达下降,Bax、Caspase-3表达水平升高;洛铂对肝癌细胞HepG2的放疗增敏比分别是1.12、1.28。结论洛铂具有放射增敏作用;其机制可能与促进肝癌细胞凋亡以及细胞G2/M期阻滞相关。
Objective To study the radiation enhancement effect of lobaplatin on hepatoma cell HepG2 in vitro. Methods MTT was used to testify the inhibitory effect of lobaplatin on human hepatoma cell line HepG2 and IC50 was calculated. Flow cytometry was applied to detect the cell cycle distribution and cell apoptosis of two concentrations of lobaplatin （1/4 IC50 and 1/8 IC50 ）, and Westerm blot was used to observe the expressions of P, cl-2, Bax and Caspase-3. Radiosensitization enhancement ratios for lower-and higher concentrations of lobaplatin on HepG2 were determined through clonogenic survival assay. Results Hepatoma cell line HepG2 could be inhibited by lobaplatin in a concentration-dependent manner and 50% inhibiting concentration was 1.85 /Lg/ml. Elevated G2/M phase and apoptosis rate were found in the two concentrations of lobaplatin（P〈0. 05） with a decreased expression of Bcl-2 and the increased expressions of Bax and Caspase-3. Radiosensitization enhancement ratioes for low-and higher concentrations of lobaplatin were 1.12 and 1.28, respectively. Conclusion Lobaplatin could enhance the efficacy of radiation on hepatoma cell line HepG2. The possible mechanism may be associated with its promotion of aDoDtosis and G2/M arrest.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第21期2510-2513,共4页
Jiangsu Medical Journal
基金
国家自然科学基金(30970792)
