摘要
目的探讨纳米金、银颗粒对人肝癌HepG2细胞的放射增敏作用及其机制。方法选用MTT法、克隆形成法检测纳米金、银颗粒增强x射线杀伤肿瘤细胞的作用，流式细胞仪检测对细胞凋亡及细胞周期分布影响，蛋白印记法检测对细胞Caspase-3、Bax、Bcl-2蛋白表达变化，酶标仪检测对细胞过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、总谷胱甘肽（GSH）水平改变。结果纳米金、银颗粒对HepG2细胞的生长抑制作用Ic50分别为6．51、2．47μg／ml。由D0值得到1／5IC50纳米金、银颗粒放射增敏比分别为1．37、1．48，1／10 IC50的分别为1．11、1．09。纳米金、银颗粒增加Caspase-3、Bax表达，降低Bcl-2表达，减少CAT、SOD、总GSH水平。结论纳米金、银颗粒均能增加HepG2细胞的放射敏感性，其机制可能为激活线粒体凋亡通路和诱导活性氧产生凋亡。
Objective To study the radiosensitizing effect of nano-gold nano-silver particles in hepatocellular carcinoma cells （HepG2） in vitro and the possible mechanisms. Methods MTF assay and clonogenic assay were performed to determine the killing effect of nano-gold and nano-silver particles in HepG2 cells. Flow-cytometry was used to measure cell apoptosis and cell cycle distribution. Western blotting was used to measure the expression of Caspase-3, Bax and Bcl-2. ELIASA was used to determine the content of catalase （CAT）, superoxide dismutase （SOD）, and total glutathione （GSH）. Results Nano-gold and nano-silver particles inhibited the proliferation of HepG2 cells with IC50 of 6. 51 μ/ml and 2. 47 μg/ml, respectively. Nano-gold and nano-silver particles significantly enhanced the radiosensitivity of HepG2 cells. Obtained by Dq, the SER of 1/5 IC50 nano-gold and nano-silver particles were 1.37 and 1.48, and 1/10 IC50 with 1.11 and 1.09. Nano-gold and nano-silver particles increased the expression of Caspase-3 and Bax and reduced the expression of Bcl-2. CAT, SOD and total GSH were significantly reduced. Conclusions Nano-gold and nano-silver particles can enhance the radiation sensitivity of HepG2 cells. Specific sensitizing mechanism may be the activation of the mitochondrial apoptosis pathway and the induction of reactive oxygen species in apoptotic pathways, which ultimately induces apoptosis.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2012年第6期571-574,共4页
Chinese Journal of Radiation Oncology
