摘要
以东北红豆杉乙醇提取紫杉醇后的废弃物为原料,利用亚临界水技术提取活性物质红豆杉多糖。系统考察了提取条件对红豆杉多糖提取得率的影响,确定了利用亚临界水提取东北红豆杉多糖的最佳工艺条件:料液比1∶15(g∶mL),提取温度150℃,提取时间1 h,提取1次,醇沉质量分数90%。在上述工艺条件下,亚临界水提取红豆杉多糖的提取得率为3.03%。此外,将亚临界水萃取法与热水回流提取和超声波辅助提取进行了比较,结果表明,亚临界水提法提取的多糖收率最高(3.03%),分别是热水回流提取和超声提取的2.31倍和7.97倍。利用红外光谱对得到的多糖进行了初步的结构分析,结果表明红豆杉多糖为β-吡喃型的酸性多糖。
The process of sub-critical water extraction of polysaccharide from processing residues of Taxus cuspidata was studied. The influences of ratio of different factors were investigated systematically. The optimal conditions were established as follows： ratio of material to liquid 1 ： 15, extraction temperature 150 ℃ , extraction time 1 h, extracting once, ethanol concentration 90 %. Under above conditions, the extraction yield of polysaccharide was 3.03 %. In addition, the effect of sub-critical water extraction was compared with refluxing extraction and ultrasonic extraction. Results showed that sub-critical water extraction possess the highest yield, which was 2.31 times and 7.97 times of the yields by refluxing extraction and ultrasonic extraction, respectively. The structure of obtained polysaccharide was analyzed using infrared spectra. IR results showed that taxus polysaecharkle was acidic polysaccharides with β-Pyranose configuration.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2012年第5期106-110,共5页
Chemistry and Industry of Forest Products
关键词
亚临界水
加工废弃物
红豆杉多糖
高效萃取
sub-critical water
processing residues
taxus polysaccharide
extraction
